Li Hui, Zhang Nong
Department of Pathology, Shanghai Medical School, Fudan University, Shanghai 200032, China.
Zhonghua Gan Zang Bing Za Zhi. 2004 Jun;12(6):347-9.
To study on the expression and genomic imprinting status of IGF2 gene in hepatocellular carcinoma.
RT-PCR semi-quantitation analysis was used to test IGF2 in paired hepatocellular carcinoma and adjacent normal tissue. Restriction fragment length polymorphism was used to detect the imprinting status of IGF2. The relationships between the expression level of IGF2, its imprinting status, and clinical information were analyzed.
The expression pattern of IGF2 was quite variable among the various HCC cases; the expression of IGF2 mRNA on cancer tissue was significantly higher than that on paired normal tissues (t=3.695); LOI was found in 11 HCC cases.
It is supposed that the enhanced expression of IGF2 gene was involved in the carcinogenesis of HCC; LOI of IGF2 may be a prophase manifestation of hepatocellular carcinoma
研究胰岛素样生长因子2（IGF2）基因在肝细胞癌中的表达及基因组印记状态。
采用逆转录-聚合酶链反应（RT-PCR）半定量分析法检测配对的肝细胞癌组织及癌旁正常组织中IGF2的表达。采用限制性片段长度多态性方法检测IGF2的印记状态。分析IGF2表达水平、印记状态与临床信息之间的关系。
IGF2在不同肝癌病例中的表达模式差异较大；癌组织中IGF2 mRNA的表达明显高于配对的正常组织（t=3.695）；11例肝癌病例中发现了印记丢失（LOI）。
推测IGF2基因表达增强参与了肝癌的发生；IGF2的LOI可能是肝细胞癌的早期表现
