[胰岛素样生长因子II和H19在人肝细胞癌中的异常印记]
[The aberrant imprinting of insulin-like growth factor II and H19 in human hepatocellular carcinoma].
作者信息
Wu Jing, Qin Yang, Li Bo, He Wen-zhi, Sun Zhi-lin
机构信息
Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
出版信息
Sichuan Da Xue Xue Bao Yi Xue Ban. 2007 Jan;38(1):49-52.
OBJECTIVE
To investigate the allelic expression of IGF2/H19 in human hepatocellular carcinoma and to analyze the relationship between the imprint status of IGF2 and that of H19.
METHODS
Heterozygotes of IGF2 and of H19 were identified by restriction fragment length polymorphism (RFLP). Allelic expression was detected by reverse transcription-polymerase chain reaction (RT-PCR) and RFLP.
RESULTS
Forty seven point one percent (47.1%, 8 of 17) of HCC samples were demonstrated to have the gain of imprinting (GOI) of in IGF2 gene, and 45.5% (5 of 11) of HCC samples were found to have the loss of imprinting (LOI) for H19 gene. No relationship was observed between the imprinting status of IGF2 and that of H19.
CONCLUSIONS
IGF2 GOI and H19 LOI are common in HCC, and the imprinting of IGF2 could be independent from that of H19 in adult liver.
目的
研究胰岛素样生长因子2（IGF2）/H19在人类肝细胞癌中的等位基因表达，并分析IGF2和H19印记状态之间的关系。
方法
通过限制性片段长度多态性（RFLP）鉴定IGF2和H19的杂合子。采用逆转录-聚合酶链反应（RT-PCR）和RFLP检测等位基因表达。
结果
47.1%（17例中的8例）的肝癌样本显示IGF2基因存在印记增强（GOI），45.5%（11例中的5例）的肝癌样本发现H19基因存在印记丢失（LOI）。未观察到IGF2和H19的印记状态之间存在关联。
结论
IGF2 GOI和H19 LOI在肝癌中常见，且在成人肝脏中IGF2的印记可能独立于H19的印记。
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