Mendes Maria Anita, de Souza Bibiana Monson, Marques Mauricio Ribeiro, Palma Mario Sergio
CEIS-Department of Biology, IBRC-UNESP (CAT-CEPID, FAPESP), Institute of Immunological Investigations (Millennium Institute-MCT, CNPq), Rio Claro, SP 13506-900, Brazil.
Toxicon. 2004 Jul;44(1):67-74. doi: 10.1016/j.toxicon.2004.04.009.
The venom of the neotropical social wasp Agelaia pallipes pallipes was fractionated by RP-HPLC resulting in the elution of seven fractions; the last two were re-fractionated under RP-HPLC by using isocratic elution conditions and the purity of the fractions were confirmed by using ESI-MS analysis. Both fractions are constituted of peptide components, which were sequenced by Edman degradation chemistry, resulting in the following sequences: Protonectin I-L-G-T-I-L-G-L-L-K-G-L-NH(2). Agelaia-MP I-N-W-L-K-L-G-K-A-I-I-D-A-L-NH(2). Both peptides are manually synthesized on solid-phase and functionally characterized by using Wistar rats cells. Protonectin is a non-hemolytic chemotactic peptide for polymorphonucleated leukocytes (PMNL), presenting some mast cell degranulating activity and potent antimicrobial action both against Gram-positive and Gram-negative bacteria. Agelaia-MP was characterized as a hemolytic mast cell degranulator toxin, presenting a poor antimicrobial action and no chemotaxis for PMNL.
新热带群居黄蜂Agelaia pallipes pallipes的毒液通过反相高效液相色谱（RP-HPLC）进行分离，得到七个馏分；最后两个馏分在RP-HPLC下采用等度洗脱条件进行再分离，并通过电喷雾电离质谱（ESI-MS）分析确认馏分的纯度。两个馏分均由肽成分组成，通过埃德曼降解化学法对其进行测序，得到以下序列：Protonectin I-L-G-T-I-L-G-L-L-K-G-L-NH₂。Agelaia-MP I-N-W-L-K-L-G-K-A-I-I-D-A-L-NH₂。两种肽均在固相上进行人工合成，并使用Wistar大鼠细胞进行功能表征。Protonectin是一种对多形核白细胞（PMNL）具有非溶血趋化作用的肽，对肥大细胞具有一定的脱颗粒活性，对革兰氏阳性菌和革兰氏阴性菌均有强大的抗菌作用。Agelaia-MP被鉴定为一种溶血肥大细胞脱颗粒毒素，抗菌作用较弱，对PMNL无趋化作用。
