MacFarlane G D, Sackrison J L, Ersfeld D L, Miller A B, Bucklen A
Director of Research and Development, DiaSorin Inc., 1951 Northwestern Ave., P.O. Box 285, Stillwater, MN 55082-0285, USA.
J Steroid Biochem Mol Biol. 2004 May;89-90(1-5):619-20. doi: 10.1016/j.jsbmb.2004.03.041.
Analysis of laboratory samples from chronic renal failure (CRF) and end stage renal disease (ESRD) patients can be problematic. Current HPLC and RIA methods for the determination of 25 OH Vitamin D involve sample extraction. However, the differences between a normal and CRF or ESRD matrix can lead to interference or inaccuracy in non-extracted, automated methods now available. The objective of this study was to assess the accuracy of the non-extracted LIAISON 25 OH Vitamin D assay in the analysis of CRF and ESRD samples as compared against RIA as reference. Samples were collected from regional reference laboratories and analyzed in both the LIAISON 25 OH Vitamin D assay and the DiaSorin 25 OH Vitamin D RIA. By Student's t test, no significant difference was observed between the RIA values and the LIAISON values (P = 0.07 CRF; P = 0.28 ESRD). The linear regression analysis resulted in the equations: CRF: LIAISON = 0.91 (RIA) + 0.6; r = 0.82 and ESRD: LIAISON = 0.93 (RIA) - 0.6; r = 0.78. From these data we conclude that the LIAISON 25 OH Vitamin D assay correctly assesses the 25 OH Vitamin D status of CRF and ESRD patients.
对慢性肾衰竭（CRF）和终末期肾病（ESRD）患者的实验室样本进行分析可能存在问题。目前用于测定25-羟基维生素D的高效液相色谱法（HPLC）和放射免疫分析法（RIA）需要进行样本提取。然而，正常样本基质与CRF或ESRD样本基质之间的差异可能会导致现有非提取式自动化方法出现干扰或不准确的情况。本研究的目的是评估与作为参考的RIA相比，非提取式LIAISON 25-羟基维生素D检测法在分析CRF和ESRD样本时的准确性。样本取自区域参考实验室，并分别采用LIAISON 25-羟基维生素D检测法和DiaSorin 25-羟基维生素D RIA进行分析。通过学生t检验，RIA值与LIAISON值之间未观察到显著差异（CRF：P = 0.07；ESRD：P = 0.28）。线性回归分析得出以下方程：CRF：LIAISON = 0.91（RIA）+ 0.6；r = 0.82；ESRD：LIAISON = 0.93（RIA）- 0.6；r = 0.78。根据这些数据，我们得出结论，LIAISON 25-羟基维生素D检测法能够正确评估CRF和ESRD患者的25-羟基维生素D状态。
