血清25-羟维生素D测定自动化方法的评估
An evaluation of automated methods for measurement of serum 25-hydroxyvitamin D.
作者信息
Wagner Dennis, Hanwell Heather E C, Vieth Reinhold
机构信息
Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, Canada.
出版信息
Clin Biochem. 2009 Oct;42(15):1549-56. doi: 10.1016/j.clinbiochem.2009.07.013. Epub 2009 Jul 21.
OBJECTIVES
To compare two new automated assays with the well-established reference method, DiaSorin radioimmunoassay (RIA), for quantitation of serum total 25-hydroxyvitamin D [25(OH)D].
METHODS
25(OH)D from human sera (n=158) was measured using DiaSorin RIA and two automated platforms, DiaSorin "LIAISON 25 OH Vitamin D TOTAL", and Roche Modular "Vitamin D3 (25-OH)". Methods were compared by regression and Bland-Altman analyses.
RESULTS
DiaSorin LIAISON demonstrated a stronger correlation (r=0.918) and better agreement (bias=-0.88 nmol/L) with DiaSorin RIA than the Roche Modular assay (r=0.871, bias=-2.55 nmol/L). Precision ranges (CV%) for the RIA, LIAISON, and Roche Modular assays, respectively, were: within run (6.8-12.9%, 2.8-8.1%, and 1.9-5.5%), and total precision (7.4-14.5%, 7.3-17.5%, and 7.6-14.5%).
CONCLUSION
DiaSorin LIAISON displayed the best correlation and agreement with DiaSorin RIA. The DiaSorin LIAISON 25 OH Vitamin D TOTAL assay is an accurate and precise automated tool for serum total 25(OH)D determination.
目的
比较两种新的自动化检测方法与成熟的参考方法——DiaSorin放射免疫分析法（RIA），用于定量血清总25-羟基维生素D[25(OH)D]。
方法
使用DiaSorin RIA以及两个自动化平台DiaSorin“LIAISON 25 OH维生素D总检测法”和罗氏模块化“维生素D3（25-OH）”检测人血清（n = 158）中的25(OH)D。通过回归分析和Bland-Altman分析对方法进行比较。
结果
与罗氏模块化检测法（r = 0.871，偏差=-2.55 nmol/L）相比，DiaSorin LIAISON与DiaSorin RIA表现出更强的相关性（r = 0.918）和更好的一致性（偏差=-0.88 nmol/L）。RIA、LIAISON和罗氏模块化检测法的精密度范围（CV%）分别为：批内（6.8 - 12.9%、2.8 - 8.1%和1.9 - 5.5%）以及总精密度（7.4 - 14.5%、7.3 - 17.5%和7.6 - 14.5%）。
结论
DiaSorin LIAISON与DiaSorin RIA表现出最佳的相关性和一致性。DiaSorin LIAISON 25 OH维生素D总检测法是一种用于血清总25(OH)D测定的准确且精密的自动化工具。
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