集成到微流控通道中的DNA置换分析。

DNA displacement assay integrated into microfluidic channels.

作者信息

Zangmeister Rebecca A, Tarlov Michael J

机构信息

Chemical Science and Technology Laboratory, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD 20899, USA.

出版信息

Anal Chem. 2004 Jul 1;76(13):3655-9. doi: 10.1021/ac035238v.

DOI:10.1021/ac035238v

PMID:15228337

Abstract

This paper describes the development of a unique fluorescence-based DNA diagnostic microfluidic assay that does not require labeling of the target sequence prior to analysis. The assay is based on the displacement of a short sacrificial fluorescent-tagged indicator oligomer by a longer untagged target sequence as it is electrophoresed through a DNA-containing hydrogel plug immobilized in a microfluidic channel. The distinct advantages of this assay are the short sensing times, as a result of directed electrophoretic transport of target DNA to the sensing element, combined with the ability to detect nonlabeled target DNA.

摘要

本文描述了一种独特的基于荧光的DNA诊断微流控检测方法的开发，该方法在分析前无需对目标序列进行标记。该检测方法基于在通过固定在微流控通道中的含DNA水凝胶塞进行电泳时，较长的未标记目标序列取代短的牺牲性荧光标记指示寡聚物。这种检测方法的显著优点是检测时间短，这是由于目标DNA向传感元件的定向电泳传输，以及能够检测未标记的目标DNA。