Ha S-K, Choi C, Jung K, Kim J, Han D U, Ha Y, Lee S-D, Kim S-H, Chae C
Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, San 56-1, Shillim-Dong, Kwanak-Gu 151-742, Seoul, Republic of Korea.
J Vet Med B Infect Dis Vet Public Health. 2004 May;51(4):166-8. doi: 10.1111/j.1439-0450.2004.00745.x.
A total of 1002 Escherichia coli strains isolated from pre-weaned pigs with diarrhoea on 1114 swine farms were screened for the presence of the adhesin involved in diffuse adherence (AIDA) gene by polymerase chain reaction (PCR). Escherichia coli isolates that carried AIDA genes were also tested by PCR for the detection of five fimbriae (F4, F5, F6, F18 and F41), heat-stable (STa, STb) and heat-labile (LT) enterotoxin, enteroaggregative E. coli heat-stable enterotoxin 1 (EAST1), and Shiga toxin 2 oedema disease (Stx2e) genes. Twenty-three (2.3%) of the 1002 E. coli isolates carried the gene for AIDA. Among 23 isolates shown to carry genes for AIDA, three carried the AIDA gene as the only shown virulence factor. Other isolates carried other virulence factor genes in addition to AIDA. Four isolates carried genes for at least one of the fimbrial adhesins and enterotoxins. Sixteen isolates carried genes for enterotoxins only. The AIDA may represent an additional virulence determinant in pre-weaned pigs with diarrhoea.
通过聚合酶链反应（PCR）对从1114个养猪场的断奶前腹泻仔猪中分离出的1002株大肠杆菌菌株进行筛选，以检测是否存在参与弥漫性黏附（AIDA）的黏附素基因。对携带AIDA基因的大肠杆菌分离株也通过PCR进行检测，以检测五种菌毛（F4、F5、F6、F18和F41）、热稳定（STa、STb）和热不稳定（LT）肠毒素、肠聚集性大肠杆菌热稳定肠毒素1（EAST1）以及志贺毒素2水肿病（Stx2e）基因。1002株大肠杆菌分离株中有23株（2.3%）携带AIDA基因。在显示携带AIDA基因的23株分离株中，有3株携带AIDA基因作为唯一显示的毒力因子。其他分离株除AIDA外还携带其他毒力因子基因。4株分离株携带至少一种菌毛黏附素和肠毒素的基因。16株分离株仅携带肠毒素基因。AIDA可能是断奶前腹泻仔猪中的另一种毒力决定因素。
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