Ha Seung-Kwon, Choi Changsun, Chae Chanhee
Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Seoul, Kwanak-Gu 151-742, Republic of Korea.
J Vet Diagn Invest. 2003 Jul;15(4):378-81. doi: 10.1177/104063870301500414.
A total of 604 Escherichia coli strains isolated from weaned pigs with diarrhea or edema disease on 653 swine farms were screened for the presence of the adhesin involved in diffuse adherence (AIDA) gene by polymerase chain reaction (PCR). Escherichia coli isolates that carried AIDA genes were also tested by PCR for the detection of 5 fimbriae (F4, F5, F6, F18, and F41), 3 heat-stable (STa, STb, and EAST1) and 1 heat-labile (LT) enterotoxin, and Shiga toxin 2e (Stx2e) genes. Forty-five (7.5%) of the 604 E. coli isolates carried the gene for AIDA. Of these 45 isolates, 5 (11.1%) carried EAST1 genes only, 1 (2.2%) carried genes for at least one of the fimbrial adhesins, 12 (26.7%) carried genes for at least one of the toxins, and 27 (60%) carried genes for at least one of the fimbrial adhesins and toxins. Fifty-one percent of strains that carried AIDA genes carried Stx2e genes, and 40% of strains that carried AIDA genes carried F18ab. The isolation rate of enterotoxigenic E. coli strain carrying genes for AIDA was 87%, and the isolation rate of Shiga toxin-producing E. coil strain carrying genes for AIDA was 49%. AIDA may represent an important virulence determinant in pigs with postweaning diarrhea or edema disease.
通过聚合酶链反应（PCR）对从653个养猪场的断奶腹泻或水肿病仔猪中分离出的604株大肠杆菌进行筛选，以检测参与弥漫性黏附（AIDA）的黏附素基因。携带AIDA基因的大肠杆菌分离株还通过PCR检测5种菌毛（F4、F5、F6、F18和F41）、3种热稳定（STa、STb和EAST1）和1种热不稳定（LT）肠毒素以及志贺毒素2e（Stx2e）基因。604株大肠杆菌分离株中有45株（7.5%）携带AIDA基因。在这45株分离株中，5株（11.1%）仅携带EAST1基因，1株（2.2%）携带至少一种菌毛黏附素基因，12株（26.7%）携带至少一种毒素基因，27株（60%）携带至少一种菌毛黏附素和毒素基因。携带AIDA基因的菌株中有51%携带Stx2e基因，携带AIDA基因的菌株中有40%携带F18ab。携带AIDA基因的产肠毒素大肠杆菌菌株的分离率为87%，携带AIDA基因的产志贺毒素大肠杆菌菌株的分离率为49%。AIDA可能是断奶后腹泻或水肿病仔猪的一个重要毒力决定因素。
