从斯洛伐克患腹泻仔猪分离出的大肠杆菌的血清型、毒力基因、紧密素类型及脉冲场凝胶电泳图谱
Serotypes, virulence genes, intimin types and PFGE profiles of Escherichia coli isolated from piglets with diarrhoea in Slovakia.
作者信息
Vu-Khac H, Holoda E, Pilipcinec E, Blanco M, Blanco J E, Dahbi G, Mora A, López C, González E A, Blanco J
机构信息
Institute of Microbiology and Immunology, Department of Food Hygiene and Technology, University of Veterinary Medicine, Komenskeho 73, Slovakia.
出版信息
Vet J. 2007 Jul;174(1):176-87. doi: 10.1016/j.tvjl.2006.05.019. Epub 2006 Sep 7.
Two hundred and fifty Escherichia coli isolates from diarrhoeic and healthy piglets were serotyped and tested for the presence of virulence genes for fimbriae, intimin, heat-labile (LT) and heat-stable (STa and STb) enterotoxins, Stx toxins, and enteroaggregative heat-stable 1 (EAST1) enterotoxin by polymerase chain reaction (PCR). Although 220 isolates from diarrhoeic piglets belonged to 43 O serogroups and 77 O:H serotypes, 60% were of one of the 10 serogroups O2, O8, O15, O54, O84, O101, O141, O147, O149 and O157, and 60% belonged to only 10 serotypes (O8:H-, O54:H-, O84:H7, O101:H-, O141:H-, O141:H4, O147:H-, O149:H10, O163:H-, and ONT:H-). PCR showed that 79% of 220 isolates carried genes for at least one of the virulence factors tested. The gene encoding for EAST1 was the most prevalent (65%) followed by those encoding for STb (49%), LT (42%), STa (13%), and Stx2e (4%). Eighty-three (38%) of the 220 E. coli isolates carried the gene for F4 (K88), whereas genes for F18, F5 (K99), F41, F6 (P987), F17, and intimin (eae) were detected in 9%, 3%, 3%, 3%, 1%, and 3%, respectively. Seropathotype O149:H10:F4:LT/STb/EAST1 (70 isolates) was the most common, representing 32% of isolates. Pulsed-field gel electrophoresis (PFGE) analysis with XbaI of 15 O149:H10 representative isolates from diarrhoeic piglets distinguished 14 types. The 15 isolates exhibited a wide variability of distinct restriction patterns though all belonged to the same serotype (O149:H10), and all but one showed identical virulence determinants (F4, LT, STb, and EAST1). Among 30 isolates from healthy piglets only two virulence genes were detected: EAST1 (26%) and eae (17%). In total, 12 isolates were positives for the eae gene: five isolates had intimin beta1, four possessed intimin theta and three showed intimin type xiB. This is believed to be the first study describing the presence of intimin type xiB in E. coli of porcine origin.
对250株来自腹泻和健康仔猪的大肠杆菌进行血清分型，并通过聚合酶链反应（PCR）检测其是否存在菌毛、紧密黏附素、不耐热（LT）和耐热（STa和STb）肠毒素、志贺毒素（Stx）以及聚集性耐热肠毒素1（EAST1）等毒力基因。尽管来自腹泻仔猪的220株分离株属于43个O血清群和77个O:H血清型，但60%属于10个血清群（O2、O8、O15、O54、O84、O101、O141、O147、O149和O157）之一，且60%仅属于10个血清型（O8:H-、O54:H-、O84:H7、O101:H-、O141:H-、O141:H4、O147:H-、O149:H10、O163:H-和ONT:H-）。PCR结果显示，220株分离株中有79%携带至少一种所检测毒力因子的基因。编码EAST1的基因最为普遍（65%），其次是编码STb的基因（49%）、LT的基因（42%）、STa的基因（13%）和Stx2e的基因（4%）。220株大肠杆菌分离株中有83株（38%）携带F4（K88）基因，而F18、F5（K99）、F41、F6（P987）、F17和紧密黏附素（eae）基因的检出率分别为9%、3%、3%、3%、1%和3%。血清致病型O149:H10:F4:LT/STb/EAST1（70株分离株）最为常见，占分离株的32%。对来自腹泻仔猪的15株O149:H10代表性分离株进行XbaI脉冲场凝胶电泳（PFGE）分析，区分出14种类型。这15株分离株虽然都属于同一血清型（O149:H10），但其限制性酶切图谱差异很大，除一株外，其余所有分离株均显示相同的毒力决定簇（F4、LT STb和EAST1）。在30株来自健康仔猪的分离株中，仅检测到两种毒力基因：EAST1（26%）和eae（17%）。总共12株分离株eae基因呈阳性：5株具有β1型紧密黏附素，4株具有θ型紧密黏附素，3株显示xiB型紧密黏附素。据信，这是首次描述猪源大肠杆菌中存在xiB型紧密黏附素的研究。
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