Barszczewska B, Jaroszewski J J
Section of Pharmacology, Department of Pathology and Pharmacology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland.
Pol J Vet Sci. 2004;7(2):83-90.
The aim of the present study was to investigate the influence of nitric oxide (NO) on the contractile activity of the isolated porcine ovarian and uterine arteries. Segments of the vessels, obtained from the pigs on days 1-5, 8-13 and 17-20 of the oestrous cycle, were mounted in the organ bath with Krebs-Ringer solution and contractile activity changes of the vessels were measured using isometric transducers. In Experiment I the arteries pretreated with norepinephrine (NE; 10(-7) M) were treated with sodium nitroprusside (SNP, 10(-8)-10(-4) M), a NO donor. In Experiment II administration of NE (10(-7) M) was preceded by treatment with Nomega-nitro-L-arginine methyl ester (L-NAME, 10(-8)-10(-6) M), an inhibitor of NO synthase. Donor of NO at doses of 10(-8)-10(-7) M did not affect (P>0.05) the contractility, while at doses of 10(-5)-10(-4) M caused a dose-dependent relaxation (P<0.05) of both ovarian and uterine arteries in all periods examined. Moreover, SNP at doses of 10(-6)-10(-4) M it caused significantly higher (P<0.05) relaxation of the ovarian arteries collected on days 8-13 as compared to the vessels from days 1-5 of the cycle. Pretreatment of the vessels with L-NAME caused a dose-dependent, significant (P<0.05) increase in the vasocontractile action of NE in both the ovarian and uterine arteries as compared to contractile activity of NE administered alone. Moreover, L-NAME pretreatment at a dose of 10(-6) M caused significantly higher (P<0.05) intensification of NE action in ovarian and uterine arteries collected on days 8-13 as compared to the vessels from days 1-5 (P<0.05) and 17-20 (P<0.05) of the oestrous cycle. Obtained results indicate that NO plays an important role in the regulation of the contractile activity of the isolated porcine ovarian and uterine arteries. Our data suggest that this action may be, at least in a part, dependent on the hormonal status of the organism.
本研究的目的是探讨一氧化氮（NO）对离体猪卵巢动脉和子宫动脉收缩活性的影响。从发情周期第1 - 5天、8 - 13天和17 - 20天的猪身上获取血管段，将其置于含有Krebs - Ringer溶液的器官浴槽中，使用等长换能器测量血管的收缩活性变化。在实验I中，用去甲肾上腺素（NE；10(-7) M）预处理后的动脉再用NO供体硝普钠（SNP，10(-8) - 10(-4) M）处理。在实验II中，在给予NE（10(-7) M）之前先用NO合酶抑制剂Nω-硝基-L-精氨酸甲酯（L-NAME，10(-8) - 10(-6) M）处理。剂量为10(-8) - 10(-7) M的NO供体对收缩性无影响（P>0.05），而剂量为10(-5) - 10(-4) M时，在所有检查时期均引起卵巢动脉和子宫动脉的剂量依赖性舒张（P<0.05）。此外，与发情周期第1 - 5天的血管相比，剂量为10(-6) - 10(-4) M的SNP对第8 - 13天采集的卵巢动脉引起的舒张作用明显更强（P<0.05）。与单独给予NE的收缩活性相比，用L-NAME预处理血管导致卵巢动脉和子宫动脉中NE的血管收缩作用呈剂量依赖性显著增强（P<0.05）。此外，与发情周期第1 - 5天（P<0.05）和17 - 20天（P<0.05）采集的血管相比，剂量为10(-6) M的L-NAME预处理对第8 - 13天采集的卵巢动脉和子宫动脉中NE作用的增强作用明显更强（P<0.0,5）。所得结果表明，NO在离体猪卵巢动脉和子宫动脉收缩活性的调节中起重要作用。我们的数据表明，这种作用可能至少部分取决于机体的激素状态。
