de Oliveira Walmar Roncalli P, Rady Peter L, Grady James, Hughes Thomas K, Neto Cyro F, Rivitti Evandro A, Tyring Stephen K
Department of Dermatology, University of São Paulo, São Paulo, Brazil.
Int J Dermatol. 2004 Jul;43(7):489-93. doi: 10.1111/j.1365-4632.2004.02289.x.
The presence of arginine at codon 72 in p53 protein is proposed to be a genetic risk factor in human papillomavirus (HPV)-related carcinogenesis.
To study the prevalence of p53 polymorphism at codon 72 in skin biopsies of epidermodysplasia verruciformis (EV) patients compared to DNA samples from healthy individuals.
DNA samples extracted from normal skin and tumor biopsies of 22 Brazilian patients with EV and blood samples from 27 healthy Brazilian individuals were studied for p53 codon 72 polymorphisms using restriction fragment length polymorphism (RFLP) analysis.
All EV patients with the malignant form of EV were homozygous for arginine (Arg/Arg) at codon 72 of the p53 gene, in contrast to none with the benign form (P < 0.0001).
p53 arginine polymorphism is likely to be associated with the development of skin malignancies in EV patients from Brazil.
p53蛋白第72密码子处精氨酸的存在被认为是人类乳头瘤病毒（HPV）相关致癌作用中的一个遗传风险因素。
与健康个体的DNA样本相比，研究疣状表皮发育不良（EV）患者皮肤活检中p53第72密码子多态性的患病率。
采用限制性片段长度多态性（RFLP）分析，对22例巴西EV患者的正常皮肤和肿瘤活检组织以及27例巴西健康个体的血液样本提取的DNA样本进行p53第72密码子多态性研究。
所有患有恶性EV的患者在p53基因第72密码子处均为精氨酸纯合子（Arg/Arg），而良性EV患者均无此情况（P < 0.0001）。
p53精氨酸多态性可能与巴西EV患者皮肤恶性肿瘤的发生有关。
