Kiupel M, Webster J D, Kaneene J B, Miller R, Yuzbasiyan-Gurkan V
Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing 48824, USA.
Vet Pathol. 2004 Jul;41(4):371-7. doi: 10.1354/vp.41-4-371.
Cutaneous mast cell tumors (MCTs) are one of the most common tumors in dogs. Currently, prognostic and therapeutic determinations for MCTs are primarily based on the histologic grade of the tumor, but a vast majority of MCTs are of an intermediate grade, and the prognostic relevance is highly questioned. A more detailed prognostic evaluation, especially of grade 2 canine MCTs, is greatly needed. To evaluate the prognostic significance of KIT and tryptase expression patterns in canine cutaneous MCTs, we studied 100 cutaneous MCTs from 100 dogs that had been treated with surgery only. The total survival and disease-free survival time and the time to local or distant recurrence of MCTs were recorded for all dogs. Using immunohistochemistry, 98 of these MCTs were stained with anti-KIT and antitryptase antibodies. Three KIT- and three tryptase-staining patterns were identified. The KIT-staining patterns were identified as 1) membrane-associated staining, 2) focal to stippled cytoplasmic staining with decreased membrane-associated staining, and 3) diffuse cytoplasmic staining. The tryptase-staining patterns were identified as 1) diffuse cytoplasmic staining, 2) stippled cytoplasmic staining, and 3) little to no cytoplasmic staining. Based on univariate and multivariate survival analysis, increased cytoplasmic KIT staining was significantly associated with an increased rate of local recurrence and a decreased survival rate. The tryptase-staining patterns were not significantly associated with any survival parameter. On the basis of these results, we propose a new prognostic classification of canine cutaneous MCTs, according to their KIT-staining pattern, that can be used for the routine prognostic evaluation of canine cutaneous MCTs.
皮肤肥大细胞瘤（MCTs）是犬类最常见的肿瘤之一。目前，MCTs的预后和治疗判定主要基于肿瘤的组织学分级，但绝大多数MCTs为中级，其预后相关性备受质疑。因此，非常需要进行更详细的预后评估，尤其是对2级犬类MCTs的评估。为了评估KIT和类胰蛋白酶表达模式在犬皮肤MCTs中的预后意义，我们研究了100只仅接受手术治疗的犬的100个皮肤MCTs。记录了所有犬的总生存期、无病生存期以及MCTs局部或远处复发的时间。使用免疫组织化学方法，对其中98个MCTs进行了抗KIT和抗类胰蛋白酶抗体染色。识别出了三种KIT染色模式和三种类胰蛋白酶染色模式。KIT染色模式被确定为：1）膜相关染色；2）局灶性至点状细胞质染色伴膜相关染色减少；3）弥漫性细胞质染色。类胰蛋白酶染色模式被确定为：1）弥漫性细胞质染色；2）点状细胞质染色；3）几乎无或无细胞质染色。基于单变量和多变量生存分析，细胞质KIT染色增加与局部复发率增加和生存率降低显著相关。类胰蛋白酶染色模式与任何生存参数均无显著相关性。基于这些结果，我们根据KIT染色模式提出了一种新的犬皮肤MCTs预后分类方法，可用于犬皮肤MCTs的常规预后评估。
