Ginoudis Argyrios, Pardali Dimitra, Mylonakis Mathios E, Tamvakis Androniki, Tvarijonaviciute Asta, Lymperaki Evgenia, Ceron Jose Joaquin, Polizopoulou Zoe
Diagnostic Laboratory, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 546 27 Thessaloniki, Greece.
Companion Animal Clinic, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 546 27 Thessaloniki, Greece.
Antioxidants (Basel). 2024 Nov 29;13(12):1473. doi: 10.3390/antiox13121473.
Mast cell tumors (MCTs) are common skin neoplasms in dogs. Prognostic indicators include histologic grade, clinical stage, high Ki-67 index, elevated argyrophilic nucleolus organizer regions (AgNOR) index, mutations, and recurrence after surgery. Blood serum redox status has been shown to correlate with prognostic factors in canine lymphoma and mammary tumors. This study aimed to assess the correlation between established prognostic factors and serum redox status and lipid metabolism analytes in dogs with MCTs. Dogs with cutaneous ( = 33) or subcutaneous ( = 6) MCTs, without comorbidities, were studied. Staging was evaluated based on cytology of regional lymph nodes and ultrasound-guided liver and spleen aspiration cytology. Histologic grading and immunohistochemical staining for Ki-67 and KIT patterns were performed on excised tumor specimens. Dogs were categorized by Patnaik grading (1-3), Kiupel grading (low/high), metastatic status, Ki-67 positive nuclei per cm (>23 or ≤23), and KIT pattern (I, II-III). Paraoxonase-1, Butyrylcholinesterase, Cupric Reducing Antioxidant Capacity (CUPRAC), Diacron Reactive Oxygen Metabolites (d-ROMs), and oxy-adsorbent levels were measured before any therapeutic intervention. ANOVA and independent -tests were used to detect differences in the mean values among groups. Paraoxonase-1 activity was significantly lower in Patnaik grade 3 ( = 0.003) and Kiupel high-grade ( = 0.022) MCTs. No significant differences were found in CUPRAC, d-ROMs, or oxy-adsorbent levels across different prognostic groups. This study found a significant correlation between histologic grading and Paraoxonase-1 activity, suggesting a potential role of Paraoxonase-1 as a prognostic biomarker in canine MCTs.
肥大细胞瘤（MCTs）是犬类常见的皮肤肿瘤。预后指标包括组织学分级、临床分期、高Ki-67指数、嗜银核仁组织区（AgNOR）指数升高、突变以及术后复发。血清氧化还原状态已被证明与犬淋巴瘤和乳腺肿瘤的预后因素相关。本研究旨在评估已确定的预后因素与患有MCTs的犬血清氧化还原状态和脂质代谢分析物之间的相关性。对患有皮肤（n = 33）或皮下（n = 6）MCTs且无合并症的犬进行了研究。基于区域淋巴结的细胞学检查以及超声引导下肝脏和脾脏穿刺细胞学检查进行分期评估。对切除的肿瘤标本进行组织学分级以及Ki-67和KIT模式的免疫组织化学染色。根据帕特奈克分级（1 - 3级）、基佩尔分级（低/高）、转移状态、每平方厘米Ki-67阳性核数（>23或≤23）以及KIT模式（I、II - III）对犬进行分类。在任何治疗干预之前测量对氧磷酶-1、丁酰胆碱酯酶、铜还原抗氧化能力（CUPRAC）、戴克隆活性氧代谢物（d-ROMs）和氧吸附剂水平。使用方差分析和独立t检验来检测各组平均值之间的差异。对氧磷酶-1活性在帕特奈克3级（P = 0.003）和基佩尔高级别（P = 0.022）MCTs中显著降低。在不同预后组的CUPRAC、d-ROMs或氧吸附剂水平上未发现显著差异。本研究发现组织学分级与对氧磷酶-1活性之间存在显著相关性，表明对氧磷酶-1作为犬MCTs预后生物标志物具有潜在作用。
