Ellerbroek L, Lox C
Bundesinstitut für Risikobewertung, Berlin.
Dtsch Tierarztl Wochenschr. 2004 May;111(5):181-4.
In this study comparative investigations of the total viable counts of bacteria and viable counts of Enterobacteriaceae with the ATP content were carried out on 70 poultry carcasses and neck skins thereof. The results revealed a correlation of total viable counts on the neck skin and on the carcass sample. Therefore the examination on total viable counts of neck skin is able to give results on the respective load of the carcass (coefficient of correlation (r) = 0.85 and coefficient of determination (R2) = 0.71). This only applies, however, to a slaughter technology at which the poultry hangs up site down with the neck skin to the bottom. The results of the ATP investigation expressed in Relative Light Units (RLU) in a bioluminescence method and total viable counts of neck skin samples are correlated (r = 0.85; R2 = 0.72). Lower values for r and R2 were estimated for RLU/Enterobacteriaceae on neck skin (r = 0.64; R2 = 0.41), for RLU/total viable counts on the carcass (r = 0.66; R2 = 0.46) and for RLU/Enterobacteriaceae on the carcass (r = 0.33; R2 = 0.11). To estimate the hygiene status in poultry slaughtery, sampling from the neck skin can replace the sampling on the whole carcass The bioluminescence method is suitable to replace the determination of total viable counts in the context of in-house hygiene supervision. However, this method seemed to be less reliable to predict the Enterobacteriaceae counts.
本研究对70个家禽胴体及其颈部皮肤进行了细菌总活菌数、肠杆菌科活菌数与ATP含量的对比研究。结果显示颈部皮肤和胴体样本的总活菌数之间存在相关性。因此,对颈部皮肤总活菌数的检测能够得出胴体相应的污染程度(相关系数(r)=0.85,决定系数(R2)=0.71)。然而,这仅适用于家禽倒挂、颈部皮肤朝下的屠宰工艺。采用生物发光法以相对光单位(RLU)表示的ATP检测结果与颈部皮肤样本的总活菌数相关(r = 0.85; R2 = 0.72)。颈部皮肤RLU/肠杆菌科(r = 0.64; R2 = 0.41)、胴体RLU/总活菌数(r = 0.66; R2 = 0.46)以及胴体RLU/肠杆菌科(r = 0.33; R2 = 0.11)的r和R2值较低。为评估家禽屠宰场的卫生状况,采集颈部皮肤样本可替代对整个胴体的采样。在内部卫生监督中,生物发光法适用于替代总活菌数的测定。然而,该方法在预测肠杆菌科菌数方面似乎不太可靠。