Siragusa G R, Dorsa W J, Cutter C N, Perino L J, Koohmaraie M
United States Department of Agriculture, Agricultural Research Service (USDA-ARS), Roman L. Hruska US Meat Animal Research Center, Clay Center, NE 68933, USA.
J Biolumin Chemilumin. 1996 Nov-Dec;11(6):297-301. doi: 10.1002/(SICI)1099-1271(199611)11:6<297::AID-BIO422>3.0.CO;2-C.
A newly developed rapid microbial ATP bioluminescence test (R-mATP) was shown to be an adequate means to assay the microbial load of poultry carcasses. This assay utilizes differential extraction and filtration to separate somatic from microbial ATP in a very rapid timeframe. The assay requires approximately 5 min to complete; approximately 3.5 min to sample and 90 s analytical time. Correlation coefficient (r) between aerobic colony counts and R-mATP test results (n=329) was 0.82. Post-test probabilities to correctly classify carcasses with different levels of microbial contamination were as high as 98% for samples of > or = 3.5 log aerobic CFU per ml. Given the rapidity of this assay, the R-mATP holds potential for monitoring the microbial load of carcasses at poultry-processing critical control points. Other potential applications of this new version of the microbial ATP bioluminescence test are discussed.
一种新开发的快速微生物ATP生物发光测试（R-mATP）被证明是测定家禽胴体微生物负荷的一种适当方法。该测试利用差异提取和过滤在非常短的时间内将体细胞ATP与微生物ATP分离。该测试大约需要5分钟完成；采样约3.5分钟，分析时间90秒。需氧菌落计数与R-mATP测试结果之间的相关系数（r）（n = 329）为0.82。对于每毫升需氧CFU≥3.5 log的样本，正确分类不同微生物污染水平胴体的测试后概率高达98%。鉴于该测试的快速性，R-mATP在监测家禽加工关键控制点的胴体微生物负荷方面具有潜力。本文还讨论了这种新版本的微生物ATP生物发光测试的其他潜在应用。
