鬼针草水浸剂抗氧化及免疫调节活性的体外研究

In vitro study of the antioxidant and immunomodulatory activity of aqueous infusion of Bidens pilosa.

作者信息

Abajo Celia, Boffill María Angeles, del Campo Jaime, Alexandra Méndez María, González Yisel, Mitjans Montserrat, Pilar Vinardell María

机构信息

Department of Fisiologia-Divisió, IV, Facultat de Farmàcia, Av. Joan XXIII s/n, E-08028 Barcelona, Spain.

出版信息

J Ethnopharmacol. 2004 Aug;93(2-3):319-23. doi: 10.1016/j.jep.2004.03.050.

DOI:10.1016/j.jep.2004.03.050

PMID:15234771

Abstract

Bidens pilosa is an annual plant from tropical America with anti-inflammatory properties in hepatitis, laryngitis, headache and digestive disorders, among others. Its wide pharmacological applications can be attributed to its chemical composition, with inhibitory effects on pathogenic microorganisms and flavonoids, which show strong antioxidant capacities. We investigated the antioxidant activity of an aqueous infusion of Bidens pilosa by studying its protective effect on the hemolysis induced by an initiator of radicals such as 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH). The immunomodulatory activity of the infusion was tested using whole blood cells. Cytokine production increased in whole blood stimulated or not by lipopolysaccharides (LPSs). The infusion is also characterized by its capacity to protect erythrocytes from the phototoxic effect of chlorpromazine, which allows its use as a potential photoprotector. Finally, it did not show ocular irritation, as demonstrated by the effect on hemoglobin denaturation. This study supports the health benefits of the ingestion of the infusion.

摘要

三叶鬼针草是一种来自热带美洲的一年生植物，对肝炎、喉炎、头痛和消化系统疾病等具有抗炎特性。其广泛的药理应用可归因于其化学成分，对致病微生物和具有强大抗氧化能力的黄酮类化合物具有抑制作用。我们通过研究三叶鬼针草水浸液对由自由基引发剂如2,2'-偶氮二异丁脒二盐酸盐（AAPH）诱导的溶血的保护作用，来研究其抗氧化活性。使用全血细胞测试浸液的免疫调节活性。在有或没有脂多糖（LPS）刺激的全血中，细胞因子的产生都会增加。该浸液的另一个特点是能够保护红细胞免受氯丙嗪的光毒性作用，这使其有潜力用作光保护剂。最后，如对血红蛋白变性的影响所示，它没有表现出眼部刺激性。这项研究支持了饮用该浸液对健康有益的观点。