（18）18F - FDG在动脉粥样硬化斑块中的积聚：免疫组织化学和PET成像研究。
(18)F-FDG accumulation in atherosclerotic plaques: immunohistochemical and PET imaging study.
作者信息
Ogawa Mikako, Ishino Seigo, Mukai Takahiro, Asano Daigo, Teramoto Noboru, Watabe Hiroshi, Kudomi Nobuyuki, Shiomi Masashi, Magata Yasuhiro, Iida Hidehiro, Saji Hideo
机构信息
Department of Investigative Radiology, Research Institute, National Cardiovascular Center, Suita, Japan.
出版信息
J Nucl Med. 2004 Jul;45(7):1245-50.
UNLABELLED
The rupture of atherosclerotic plaques and the subsequent formation of thrombi are the main factors responsible for myocardial and cerebral infarctions. Thus, the detection of vulnerable plaques in atherosclerotic lesions is a desirable goal, and attempts to image these plaques with (18)F-FDG have been made. In the present study, the relationship between the accumulation of (18)F-FDG and the biologic characteristics of atherosclerotic lesions was investigated. Furthermore, PET imaging of vulnerable plaques was performed with an animal model of atherosclerosis, Watanabe heritable hyperlipidemic (WHHL) rabbits.
METHODS
WHHL (n = 11) and control (n = 3) rabbits were injected intravenously with (18)F-FDG, and the thoracic and abdominal aortas were removed 4 h after injection. The accumulated radioactivity was measured, and the number of macrophages and the intimal area were investigated by examination of stained sections. PET and CT images were also acquired at 210 min after injection of the radiotracer.
RESULTS
(18)F-FDG accumulated to a significantly higher level in the aortas of the WHHL rabbits (mean +/- SD differential uptake ratio [DUR], 1.47 +/- 0.90) than in those of the control rabbits (DUR, 0.44 +/- 0.15); DUR was calculated as (tissue activity/tissue weight)/(injected radiotracer activity/animal body weight), with activities given in becquerels and weights given in kilograms. (18)F-FDG uptake and the number of macrophages were strongly correlated in the atherosclerotic lesions of the WHHL rabbits (R = 0.81). In the PET analysis, intense (18)F-FDG radioactivity was detected in the aortas of the WHHL rabbits, whereas little radioactivity was seen in the control rabbits.
CONCLUSION
The results suggest that macrophages are responsible for the accumulation of (18)F-FDG in atherosclerotic lesions. Because vulnerable plaques are rich in macrophages, (18)F-FDG imaging should be useful for the selective detection of such plaques.
未标注
动脉粥样硬化斑块破裂及随后血栓形成是导致心肌梗死和脑梗死的主要因素。因此，检测动脉粥样硬化病变中的易损斑块是一个理想目标，人们已尝试用（18）F-FDG对这些斑块进行成像。在本研究中，研究了（18）F-FDG蓄积与动脉粥样硬化病变生物学特性之间的关系。此外，还利用动脉粥样硬化动物模型——渡边遗传性高脂血症（WHHL）兔对易损斑块进行了PET成像。
方法
对11只WHHL兔和3只对照兔静脉注射（18）F-FDG，注射后4小时取出胸主动脉和腹主动脉。测量蓄积的放射性，并通过检查染色切片研究巨噬细胞数量和内膜面积。在注射放射性示踪剂后210分钟也采集了PET和CT图像。
结果
（18）F-FDG在WHHL兔主动脉中的蓄积水平（平均±标准差差异摄取率[DUR]，1.47±0.90）显著高于对照兔（DUR，0.44±0.15）；DUR计算为（组织活性/组织重量）/（注射的放射性示踪剂活性/动物体重），活性单位为贝可勒尔，重量单位为千克。在WHHL兔的动脉粥样硬化病变中，（18）F-FDG摄取与巨噬细胞数量密切相关（R = 0.81）。在PET分析中，在WHHL兔主动脉中检测到强烈的（18）F-FDG放射性，而在对照兔中几乎未见放射性。
结论
结果表明巨噬细胞是（18）F-FDG在动脉粥样硬化病变中蓄积的原因。由于易损斑块富含巨噬细胞，（18）F-FDG成像应有助于选择性检测此类斑块。
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