在没有¹³C射频通道的情况下，使用¹³C标记葡萄糖对猴子大脑氧化代谢进行核磁共振测量。

NMR measurement of brain oxidative metabolism in monkeys using 13C-labeled glucose without a 13C radiofrequency channel.

作者信息

Boumezbeur Fawzi, Besret Laurent, Valette Julien, Vaufrey Françoise, Henry Pierre-Gilles, Slavov Velislav, Giacomini Eric, Hantraye Philippe, Bloch Gilles, Lebon Vincent

机构信息

CEA-SHFJ, Orsay, France.

出版信息

Magn Reson Med. 2004 Jul;52(1):33-40. doi: 10.1002/mrm.20129.

DOI:10.1002/mrm.20129

PMID:15236364

Abstract

We detected glutamate C4 and C3 labeling in the monkey brain during an infusion of [U-13C6]glucose, using a simple 1H PRESS sequence without 13C editing or decoupling. Point-resolved spectroscopy (PRESS) spectra revealed decreases in 12C-bonded protons, and increases in 13C-bonded protons of glutamate. To take full advantage of the simultaneous detection of 12C- and 13C-bonded protons, we implemented a quantitation procedure to properly measure both glutamate C4 and C3 enrichments. This procedure relies on LCModel analysis with a basis set to account for simultaneous signal changes of protons bound to 12C and 13C. Signal changes were mainly attributed to 12C- and 13C-bonded protons of glutamate. As a result, we were able to measure the tricarboxylic acid (TCA) cycle flux in a 3.9 cm3 voxel centered in the monkey brain on a whole-body 3 Tesla system (VTCA = 0.55 +/- 0.04 micromol x g(-1) x min(-1), N = 4). This work demonstrates that oxidative metabolism can be quantified in deep structures of the brain on clinical MRI systems, without the need for a 13C radiofrequency (RF) channel.

摘要

我们在输注[U-13C6]葡萄糖期间，使用简单的无13C编辑或去耦的1H PRESS序列，在猴脑中检测到谷氨酸C4和C3标记。点分辨光谱（PRESS）谱显示与12C键合的质子减少，而谷氨酸中与13C键合的质子增加。为了充分利用对与12C和13C键合的质子的同时检测，我们实施了一种定量程序来准确测量谷氨酸C4和C3的富集情况。该程序依赖于基于LCModel分析的基集，以解释与12C和13C结合的质子的同时信号变化。信号变化主要归因于谷氨酸中与12C和13C键合的质子。结果，我们能够在全身3特斯拉系统上，测量以猴脑为中心的3.9立方厘米体素中的三羧酸（TCA）循环通量（VTCA = 0.55±0.04微摩尔×克-1×分钟-1，N = 4）。这项工作表明，无需13C射频（RF）通道，在临床MRI系统上即可对脑深部结构的氧化代谢进行定量分析。