酿酒酵母中Adr1依赖性基因的常见染色质结构、常见染色质重塑和常见转录动力学。

Common chromatin architecture, common chromatin remodeling, and common transcription kinetics of Adr1-dependent genes in Saccharomyces cerevisiae.

作者信息

Agricola Eleonora, Verdone Loredana, Xella Barbara, Di Mauro Ernesto, Caserta Micaela

机构信息

Fondazione Istituto Pasteur-Fondazione Cenci Bolognetti, c/o Dipartimento di Genetica e Biologia Molecolare, Università La Sapienza, 185 Rome, Italy.

出版信息

Biochemistry. 2004 Jul 13;43(27):8878-84. doi: 10.1021/bi049577+.

DOI:10.1021/bi049577+

PMID:15236596

Abstract

The chromatin structure of several Saccharomyces cerevisiae ADR1-dependent genes was comparatively analyzed in vivo in order to evaluate the role of promoter architecture in transcriptional control. In repressing conditions (high glucose) a nucleosome particle always obstructs the TATA box, immediately adjacent to an upstream-located nucleosome-free region containing a cluster of Adr1 binding sites. Upon derepression the TATA box-containing nucleosome is destabilized according to a mechanism shared by all of the genes studied. The transcription factor Adr1 is always required for the observed chromatin remodeling. mRNA accumulation of all of the genes analyzed is strongly delayed in the absence of the acetyltransferase Gcn5 and is decreased in the presence of a temperature-sensitive Esa1 mutant. The results suggest that a defined promoter chromatin structure, controlled by DNA conformational features, is relevant for the activation of coregulated genes.

摘要

为了评估启动子结构在转录调控中的作用，对酿酒酵母中几个依赖ADR1的基因的染色质结构进行了体内比较分析。在抑制条件下（高葡萄糖），一个核小体颗粒总是会阻碍TATA框，TATA框紧邻一个位于上游的无核小体区域，该区域包含一组Adr1结合位点。去抑制后，含TATA框的核小体根据所有研究基因共有的机制而变得不稳定。观察到的染色质重塑总是需要转录因子Adr1。在缺乏乙酰转移酶Gcn5的情况下，所有分析基因的mRNA积累都被强烈延迟，而在温度敏感的Esa1突变体存在时则会减少。结果表明，由DNA构象特征控制的特定启动子染色质结构与共调控基因的激活相关。