Du Zhengda, Deng Hong, Cheng Yanfei, Zhai Zhiguang, Guo Xuena, Wang Zhaoyue, He Xiuping
CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
College of Life Sciences, University of the Chinese Academy of Sciences, Beijing, China.
Front Microbiol. 2022 Jun 15;13:898938. doi: 10.3389/fmicb.2022.898938. eCollection 2022.
Cat8 is an important transcription factor regulating the utilization of non-fermentative carbon sources in . However, our previous studies found that Cat8 may play a critical role in nitrogen metabolism, but the regulatory mechanism has not been elucidated. In this study, the nuclear localization and analysis of regulatory activity showed that the Cat8 function relies on Snf1 kinase. In the fermentation with glucose or glycerol as carbon sources under phenylalanine (Phe) induction, by comparing the changes of cellular gene expression and Cat8 target gene binding profiles after Cat8 overexpression, enhanced transcription was shown among key genes involved in the Ehrlich pathway (e.g., , , and ) and its upstream and downstream related factors (e.g., , , , , and ), indicating that Cat8 participated in the regulation of nitrogen metabolism. Moreover, highly active Cat8 interacts with transcriptional activator Aro80 and GATA activator Gat1 coordinately to regulate the transcription of . Altogether, our results showed that Cat8 may act as a global transcription factor in response to nutritional changes, regulating both carbon and nitrogen utilization. This provides a new insight for us to explore the regulation of cell nutrient metabolism networks in yeast.
Cat8是一种重要的转录因子，可调节……中非发酵性碳源的利用。然而，我们之前的研究发现Cat8可能在氮代谢中起关键作用，但其调控机制尚未阐明。在本研究中，核定位及调控活性分析表明，Cat8的功能依赖于Snf1激酶。在苯丙氨酸（Phe）诱导下以葡萄糖或甘油作为碳源的发酵过程中，通过比较Cat8过表达后细胞基因表达的变化以及Cat8靶基因的结合图谱，发现艾氏途径（如……、……和……）及其上下游相关因子（如……、……、……、……和……）中的关键基因转录增强，表明Cat8参与了氮代谢的调控。此外，高活性的Cat8与转录激活因子Aro80和GATA激活因子Gat1协同作用，调控……的转录。总之，我们的结果表明，Cat8可能作为一种全局转录因子响应营养变化，调节碳和氮的利用。这为我们探索酵母细胞营养代谢网络的调控提供了新的见解。
