Schoenborn Liesbeth, Yates Penelope S, Grinton Bronwyn E, Hugenholtz Philip, Janssen Peter H
Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria 3010, Australia.
Appl Environ Microbiol. 2004 Jul;70(7):4363-6. doi: 10.1128/AEM.70.7.4363-4366.2004.
Representatives of only four well-characterized bacterial phyla were isolated from a pasture soil by using liquid serial dilution culture. In contrast, members of Acidobacteria, Verrucomicrobia, and Gemmatimonadetes and of other poorly represented bacterial lineages were isolated in earlier experiments with solidified versions of the same media. We conclude that, contrary to expectation, liquid serial dilution culture is inferior to culturing on solid media for isolating representatives of many bacterial phyla from soil.
通过液体连续稀释培养法,仅从一块牧场土壤中分离出了四个特征明确的细菌门的代表菌株。相比之下,在早期使用相同培养基固化版本的实验中,分离出了酸杆菌门、疣微菌门、芽单胞菌门以及其他代表性较差的细菌谱系的成员。我们得出结论,与预期相反,对于从土壤中分离许多细菌门的代表菌株而言,液体连续稀释培养法不如在固体培养基上培养。