Department of Biology, Faculty of Science, Mahasarakham University, Maha Sarakham Province, 44150, Thailand.
Department of Medical Biotechnology, College of Medicine and Public Health, Flinders University, Adelaide, Australia.
Arch Microbiol. 2021 Aug;203(6):3341-3352. doi: 10.1007/s00203-021-02308-4. Epub 2021 Apr 19.
An endophytic actinobacterium, strain CAP261 was isolated from the surface sterilized root of Callitris preissii (Australian native pine tree). As a result of a polyphasic taxonomy study, this strain was identified as a member of the genus Streptomyces. This strain was an aerobic actinobacterium with well-developed substrate mycelia with loop spore chains and the spore surfaces are verrucose. The closest phylogenetic members which shared the highest 16S rRNA gene sequences similarity was Streptomyces bottropensis ATCC 25435 at 98.1%. Chemotaxonomic data including cell wall components, major menaquinones, and major fatty acids confirmed the affiliation of strain CAP261 to the genus Streptomyces. The results of the phylogenetic analysis, including physiological and biochemical studies in combination with genome comparison study, allowed the genotypic and phenotypic differentiation of strain CAP261 and the closest species with validly published names. ANIb, ANIm and dDDH values of strain CAP261 and S. bottropensis ATCC 25435 were 86.7%, 89.2% and 33.9%, respectively. The name proposed for the new species is Streptomyces adelaidensis sp. nov. The type strain is CAP261 (= DSM 42026 = NRRL B-24814).
从经表面消毒的澳洲白千层（澳大利亚原生松树）根部分离出一株内生放线菌，菌株 CAP261。经过多相分类学研究，该菌株被鉴定为链霉菌属的一员。该菌株是一种需氧放线菌，具有发达的基质菌丝和环孢子链，孢子表面呈疣状。与该菌株 16S rRNA 基因序列相似度最高的最接近的系统发育成员是bottropensis ATCC 25435，相似度为 98.1%。化学分类学数据包括细胞壁成分、主要类异戊二烯醌和主要脂肪酸，证实了菌株 CAP261 与链霉菌属的归属。包括生理生化研究在内的系统发育分析结果以及基因组比较研究，使得菌株 CAP261 与其最接近的具有有效发表名称的种在基因型和表型上得以区分。菌株 CAP261 和 bottropensis ATCC 25435 的 ANIb、ANI 和 dDDH 值分别为 86.7%、89.2%和 33.9%。该新种的命名为阿德莱德链霉菌（Streptomyces adelaidensis）。其模式菌株为 CAP261（= DSM 42026 = NRRL B-24814）。
