Hsu Ya-Ling, Kuo Po-Lin, Liu Chi-Feng, Lin Chun-Ching
Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, No. 100, Shin-Chuan 1st Road, Kaohsiung 807, Taiwan, ROC.
Cancer Lett. 2004 Aug 20;212(1):53-60. doi: 10.1016/j.canlet.2004.02.019.
In this study, we examined acacetin (5,7-dihydroxy-4'-methoxyflavone), a flavonoid compound, for its effect on proliferation in human non-small cell lung cancer A549 cells. The results first reported that acacetin not only inhibited A549 cell proliferation but also induced apoptosis and blocked cell cycle progression in the G1 phase. ELISA assay demonstrated that acacetin significantly increased the expression of p53 and p21/WAF1 protein, which caused cell cycle arrest. An enhancement in Fas and its two forms of ligands, membrane-bound Fas ligand (mFasL) and soluble Fas ligand (sFasL), might be responsible for the apoptotic effect induced by acacetin. Taken together, p53 and Fas/FasL apoptotic system may participate in the antiproliferative activity of acacetin in A549 cells.
在本研究中，我们检测了黄酮类化合物刺槐素（5,7-二羟基-4'-甲氧基黄酮）对人非小细胞肺癌A549细胞增殖的影响。结果首次报道，刺槐素不仅抑制A549细胞增殖，还诱导细胞凋亡并使细胞周期在G1期停滞。ELISA分析表明，刺槐素显著增加p53和p21/WAF1蛋白的表达，从而导致细胞周期停滞。Fas及其两种配体形式，即膜结合型Fas配体（mFasL）和可溶性Fas配体（sFasL）的增加，可能是刺槐素诱导凋亡效应的原因。综上所述，p53和Fas/FasL凋亡系统可能参与了刺槐素对A549细胞的抗增殖活性。
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