孕期胎儿间充质干细胞迁移数十年后女性骨髓和骨骼中的微嵌合体现象。

Microchimerism in female bone marrow and bone decades after fetal mesenchymal stem-cell trafficking in pregnancy.

作者信息

O'Donoghue Keelin, Chan Jerry, de la Fuente Josu, Kennea Nigel, Sandison Ann, Anderson Jonathan R, Roberts Irene A G, Fisk Nicholas M

机构信息

Institute of Reproductive and Developmental Biology, Division of Paediatrics, Obstetrics and Gynaecology, Imperial College London, Hammersmith Campus, London W12 0NN, UK.

出版信息

Lancet. 2004;364(9429):179-82. doi: 10.1016/S0140-6736(04)16631-2.

DOI:10.1016/S0140-6736(04)16631-2

PMID:15246731

Abstract

Fetal cells enter maternal blood during pregnancy and persist in women with autoimmune disease. The frequency of subsequent fetomaternal microchimerism in healthy women and its cell type is unknown. To test the hypothesis that fetal mesenchymal stem cells persist in maternal organs, we studied female bone marrow and ribs. Male cells were identified by XY fluorescence in-situ hybridisation in marrow-derived mesenchymal stem cells and in rib sections from all women with male pregnancies, but not in controls (9/9 vs 0/5, p=0.0005). We conclude that fetal stem cells transferred into maternal blood engraft in marrow, where they remain throughout life. This finding has implications for normal pregnancy, for obstetric complications that increase fetomaternal trafficking, and for graft survival after transplantation.

摘要

孕期胎儿细胞会进入母体血液，并在患有自身免疫性疾病的女性体内持续存在。健康女性中后续胎儿-母体微嵌合体的频率及其细胞类型尚不清楚。为了验证胎儿间充质干细胞在母体器官中持续存在的假设，我们研究了女性的骨髓和肋骨。通过XY荧光原位杂交在所有怀有男性胎儿的女性的骨髓间充质干细胞和肋骨切片中鉴定出男性细胞，但在对照组中未发现（9/9对0/5，p = 0.0005）。我们得出结论，转移到母体血液中的胎儿干细胞会在骨髓中植入，并在那里终生留存。这一发现对正常妊娠、增加胎儿-母体细胞交换的产科并发症以及移植后的移植物存活具有重要意义。