Nencioni Ana Leonor A, Lebrun Ivo, Dorce Valquiria A C
Laboratory of Pharmacology, Butantan Institute, Av. Dr. Vital Brasil, 1500, 05503-900 São Paulo, SP, Brazil.
Toxicon. 2004 Aug;44(2):179-83. doi: 10.1016/j.toxicon.2004.05.018.
We examined the effects of dantrolene, an inhibitor of intracellular calcium release, on alterations associated with the intrahippocampal injection of the TsTX scorpion toxin. Male Wistar rats (230-250 g) were injected with Ringer solution (1 microl; n = 6); TsTX toxin (1 microg/microl; n = 8); and dantrolene (10.0 mg/kg) plus TsTX toxin (1 microg/microl; n = 6). After injection, electroencephalographic (EEG) recordings and observation of animals behaviour were performed continuously for 4 h. One week later, animals were submitted to histopathological analysis. TsTX caused electrographic seizure expressed by moderate or intense discharges and neuronal loss in hippocampal areas in all injected animals (n = 8). Dantrolene reduced the effect of TsTX. Thus, 67% of rats (four out of six) treated with toxin and dantrolene had electrographic convulsions, but only for 30 min after injection and none of them presented neuronal damage. Dantrolene or Ringer had no effects on the EEG.
我们研究了细胞内钙释放抑制剂丹曲林对海马内注射TsTX蝎毒素相关改变的影响。雄性Wistar大鼠（230 - 250克）被注射林格液（1微升；n = 6）；TsTX毒素（1微克/微升；n = 8）；以及丹曲林（10.0毫克/千克）加TsTX毒素（1微克/微升；n = 6）。注射后，连续4小时进行脑电图（EEG）记录并观察动物行为。一周后，对动物进行组织病理学分析。TsTX在所有注射动物（n = 8）中引起脑电图癫痫发作，表现为海马区中度或强烈放电以及神经元丢失。丹曲林减轻了TsTX的作用。因此，用毒素和丹曲林治疗的大鼠中有67%（六只中的四只）出现脑电图惊厥，但仅在注射后30分钟出现，且它们均未出现神经元损伤。丹曲林或林格液对脑电图无影响。
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