Uchida Atsushi, Oka Yuichi, Aoyama Mineyoshi, Suzuki Shugo, Yokoi Takashi, Katano Hiroyuki, Mase Mitsuhito, Tada Toyohiro, Asai Kiyofumi, Yamada Kazuo
Department of Neurosurgery and Restorative Neuroscience, Nagoya City University Graduate School of Medical Sciences, Mizuho, Nagoya 467-8601, Japan.
Brain Res Mol Brain Res. 2004 Jul 26;126(2):129-36. doi: 10.1016/j.molbrainres.2004.04.009.
Myelencephalon-specific protease (MSP) is one of the serine proteases and is expressed in the central nervous system of rats. Its function and alternation in brain injury have not yet been clarified. In this study, we investigated the expression of MSP after transient middle cerebral artery occlusion (MCAO) using in situ hybridization and immunohistochemistry. In situ localization of MSP mRNA demonstrated a higher level in the corpus callosum and around the ischemic area from 12 h to 14 days after MCA reperfusion, with the peak of expression coming 3 days after reperfusion in both regions. Immunohistochemically, the expression of protein was found 1 day after reperfusion in the same brain region that was observed for mRNA. The peak was 7 days after reperfusion in both regions. Micro-autoradiography, immunostaining and double immunohistochemical labeling revealed the expression of MSP to be located mainly in the oligodendrocytes. The present results indicate that MSP may be related to the turnover of the myelin-associated proteins and the extracellular matrix proteins after transient MCAO. The activation of MSP may play a role in remodeling processes such as neurite outgrowth and remyelination.
延髓特异性蛋白酶（MSP）是丝氨酸蛋白酶之一，在大鼠中枢神经系统中表达。其在脑损伤中的功能及变化尚未阐明。在本研究中，我们采用原位杂交和免疫组化方法，研究了短暂性大脑中动脉闭塞（MCAO）后MSP的表达情况。MSP mRNA的原位定位显示，在MCA再灌注后12小时至14天，胼胝体和缺血区域周围的MSP mRNA水平较高，两个区域的表达峰值均出现在再灌注后3天。免疫组化结果显示，在与mRNA相同的脑区，再灌注1天后可检测到蛋白表达，两个区域的表达峰值均出现在再灌注后7天。微量放射自显影、免疫染色及双重免疫组化标记显示，MSP的表达主要位于少突胶质细胞。目前的结果表明，MSP可能与短暂性MCAO后髓鞘相关蛋白和细胞外基质蛋白的更新有关。MSP的激活可能在神经突生长和髓鞘再生等重塑过程中发挥作用。
