Imaki Junko, Tsuchiya Ken, Mishima Takuya, Onodera Hidetaka, Kim James I, Yoshida Kazuhiko, Ikeda Hiromi, Sakai Masaharu
Department of Anatomy, National Defense Medical College, Tokorozawa, Saitama 359-8513, Japan.
Biochem Biophys Res Commun. 2004 Aug 6;320(4):1323-7. doi: 10.1016/j.bbrc.2004.05.222.
Maf is a family of oncogenes which encodes a nuclear bZip transcription factor protein and has been originally identified from the avian oncogenic retrovirus, AS42. Maf genes have been reported to have critical roles in embryological development and cellular differentiation. In this study, in situ hybridization with (35)S-labeled antisense riboprobes was used to investigate the distribution of c-maf mRNA in balb/c mouse kidneys from 12 (E12) through 17 days (E17) of gestation and then 1 and 4 weeks after birth. Immunocytochemistry of 4-week-old mouse kidney using anti-c-maf antisera was also performed. Kidney and liver sections from c-maf knockout mice at 4 weeks were stained with hematoxylin-eosin, and their histological features were examined. Expression of c-maf mRNA was first detected on E16 in the renal proximal tubules, and it was expressed through 4 weeks after birth. In the c-maf knockout mice at 4 weeks the cytoplasmic volume of the proximal tubule and liver cell was smaller. These findings suggest that expression of the c-maf gene may be involved in the embryological development and/or cell differentiation of kidney and liver cells.
Maf是一个致癌基因家族，编码一种核bZip转录因子蛋白，最初是从禽致癌逆转录病毒AS42中鉴定出来的。据报道，Maf基因在胚胎发育和细胞分化中起关键作用。在本研究中，使用（35）S标记的反义核糖探针进行原位杂交，以研究妊娠12天（E12）至17天（E17）以及出生后1周和4周时，c-maf mRNA在balb/c小鼠肾脏中的分布。还使用抗c-maf抗血清对4周龄小鼠肾脏进行了免疫细胞化学分析。对4周龄c-maf基因敲除小鼠的肾脏和肝脏切片进行苏木精-伊红染色，并检查其组织学特征。c-maf mRNA的表达在E16时首次在肾近端小管中检测到，并在出生后4周内持续表达。在4周龄的c-maf基因敲除小鼠中，近端小管和肝细胞的细胞质体积较小。这些发现表明，c-maf基因的表达可能参与了肾脏和肝细胞的胚胎发育和/或细胞分化。
