Tissue distribution of the DNA binding oncoprotein Maf during chicken development.

To assess the normal function of the c-maf protooncogene product, we examined its tissue distribution during fetal growth and organogenesis of the chicken using an immunohistochemical technique. Nuclei of neuronal cells in the optic lobe and in the outer granular layer of the cerebral cortex were efficiently stained by anti-Maf serum throughout all developmental stages examined. In the lung, mesenchymal cells were stained intensely by the antiserum, with the most intense staining around embryonic day 15. During the embryonic period, nuclei of mesenchymal cells in the perichondrial and periosteal tissues as well as intestinal submucosa were also stained specifically by the antiserum. In addition, we detected substantial Maf immunoreactivity in nuclei of renal glomerular and proximal-tubular epithelial cells. These results suggest that c-Maf protein plays some important roles in the development of the central nervous system and tissues of mesodermal origin such as connective and renal tissues.