Nakayama H, Yamasaki H, Nishizawa M, Goto N
Department of Veterinary Pathology, University of Tokyo, Japan.
Int J Dev Biol. 1995 Dec;39(6):957-64.
To assess the normal function of the c-maf protooncogene product, we examined its tissue distribution during fetal growth and organogenesis of the chicken using an immunohistochemical technique. Nuclei of neuronal cells in the optic lobe and in the outer granular layer of the cerebral cortex were efficiently stained by anti-Maf serum throughout all developmental stages examined. In the lung, mesenchymal cells were stained intensely by the antiserum, with the most intense staining around embryonic day 15. During the embryonic period, nuclei of mesenchymal cells in the perichondrial and periosteal tissues as well as intestinal submucosa were also stained specifically by the antiserum. In addition, we detected substantial Maf immunoreactivity in nuclei of renal glomerular and proximal-tubular epithelial cells. These results suggest that c-Maf protein plays some important roles in the development of the central nervous system and tissues of mesodermal origin such as connective and renal tissues.
为评估c-maf原癌基因产物的正常功能，我们运用免疫组化技术检测了其在鸡胚胎生长和器官形成过程中的组织分布。在所检测的所有发育阶段，抗Maf血清均能有效染色视叶和大脑皮质外颗粒层中神经元细胞的细胞核。在肺中，间充质细胞被抗血清强烈染色，在胚胎第15天左右染色最为强烈。在胚胎期，软骨膜、骨膜组织以及肠黏膜下层中间充质细胞的细胞核也被抗血清特异性染色。此外，我们在肾小球和近端肾小管上皮细胞的细胞核中检测到大量的Maf免疫反应性。这些结果表明，c-Maf蛋白在中枢神经系统以及中胚层来源的组织（如结缔组织和肾脏组织）的发育中发挥着一些重要作用。
