[人类卵母细胞的第一极体无助于预测纺锤体的精确位置]
[The first polar body of human oocytes does not help predict the precise location of the spindle].
作者信息
Zhu Liang, Kong Ling-hong, Li Hong, Chen Shi-Ling, Xing Fu-qi
机构信息
Assisted Reproductive Technology Center, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
出版信息
Di Yi Jun Yi Da Xue Xue Bao. 2004 Jul;24(7):809-11.
OBJECTIVE
To observe the precise position of the metaphase II spindle for facilitating clinical practice of assisted reproduction.
METHODS
LC-PolScope imaging system was used for observing the spindles in human metaphase II oocytes matured in vitro or in vivo, and the angle between the center of the oocyte, the spindle and the first polar body was measured and compared between in vitro and in vivo matured oocytes.
RESULTS
In vitro and in vivo matured oocytes were significantly different in the angle between the spindle and the first polar body (P=0.006).
CONCLUSION
The deviation of the spindle from the polar body in in vitro matured oocyte was significantly smaller than that in in vivo matured oocyte, and the first polar body does not help predict the precise location of the metaphase II spindle. The imaging system is safe and effective for observing the spindles.
目的
观察中期II纺锤体的精确位置，以促进辅助生殖的临床实践。
方法
采用LC-PolScope成像系统观察体外或体内成熟的人中期II卵母细胞中的纺锤体，并测量并比较体外和体内成熟卵母细胞中卵母细胞中心、纺锤体与第一极体之间的角度。
结果
体外和体内成熟的卵母细胞在纺锤体与第一极体之间的角度上存在显著差异（P = 0.006）。
结论
体外成熟卵母细胞中纺锤体与极体的偏差明显小于体内成熟卵母细胞，且第一极体无助于预测中期II纺锤体的精确位置。该成像系统用于观察纺锤体安全有效。
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