Cohen Y, Malcov M, Schwartz T, Mey-Raz N, Carmon A, Cohen T, Lessing J B, Amit A, Azem F
The In Vitro Fertilization Unit, Lis Maternity Hospital, Tel-Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel.
Hum Reprod. 2004 Mar;19(3):649-54. doi: 10.1093/humrep/deh113. Epub 2004 Jan 29.
The LC Polscope facilitates visualization of the meiotic spindle in human oocyte. This study aimed to investigate meiotic spindle assembly in correlation to time elapsed after HCG administration, and to determine whether spindle imaging may serve to indicate the likelihood of fertilization and embryo cleavage.
Metaphase II (MII) oocytes from 103 couples who were being treated for male infertility were imaged with the Polscope prior to sperm injection. Spindle imaging was correlated to time elapsed from HCG administration, fertilization rate and embryo cleavage. The main outcome measures were spindle visualization, fertilization and embryo cleavage on day 3.
A total of 770 MII oocytes were imaged. A spindle was imaged in a significantly higher number of oocytes from >or=38 h after HCG administration compared with those in the <38 h group (78.1-81.5% versus 61.6%; P < 0.001). The fertilization rate in oocytes with a visible spindle was statistically higher compared with oocytes in which spindle could not be detected (70.4% versus 62.2%; P = 0.035). We found no relationship between spindle imaging and embryo cleavage on day 3.
Spindle imaging, in addition to first polar body appearance, is an accurate indicator for oocyte maturity. We suggest that spindle imaging be performed prior to sperm injection.
LC偏振光显微镜有助于观察人类卵母细胞中的减数分裂纺锤体。本研究旨在探讨减数分裂纺锤体组装与注射人绒毛膜促性腺激素（HCG）后经过的时间之间的相关性，并确定纺锤体成像是否可用于指示受精和胚胎分裂的可能性。
对103对因男性不育接受治疗的夫妇的中期II（MII）卵母细胞在精子注射前用偏振光显微镜进行成像。纺锤体成像与注射HCG后的时间、受精率和胚胎分裂情况相关。主要观察指标为第3天的纺锤体可视化、受精和胚胎分裂情况。
共对770个MII卵母细胞进行了成像。与注射HCG后<38小时组相比，注射HCG后≥38小时组的卵母细胞中成像到纺锤体的数量明显更多（78.1 - 81.5%对61.6%；P < 0.001）。可见纺锤体的卵母细胞的受精率在统计学上高于未检测到纺锤体的卵母细胞（70.4%对62.2%；P = 0.035）。我们发现纺锤体成像与第3天的胚胎分裂之间没有关系。
除了第一极体出现外，纺锤体成像也是卵母细胞成熟的准确指标。我们建议在精子注射前进行纺锤体成像。
