环氧化酶-2在人食管鳞状细胞癌中的表达。
Expression of cyclooxygenase-2 in human esophageal squamous cell carcinomas.
作者信息
Jiang Jian-Gang, Tang Jiang-Bo, Chen Chun-Lian, Liu Bao-Xing, Fu Xiang-Ning, Zhu Zhi-Hui, Qu Wei, Cianflone Katherine, Waalkes Michael-P, Wang Dao-Wen
机构信息
Department of Internal Medicine and Gene Therapy Center, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China.
出版信息
World J Gastroenterol. 2004 Aug 1;10(15):2168-73. doi: 10.3748/wjg.v10.i15.2168.
AIM
To determine whether cyclooxygenase-2 (COX-2) was expressed in human esophageal squamous cell carcinoma.
METHODS
Quantitative reverse transcription-polymerase chain reaction (RT-PCR), western blotting, immunohistoc-hemistry and immunofluorescence were used to assess the expression level of COX-2 in esophageal tissue.
RESULTS
COX-2 mRNA levels were increased by >80-fold in esophageal squamous cell carcinoma when compared to adjacent noncancerous tissue. COX-2 protein was present in 21 of 30 cases of esophageal squamous cell carcinoma tissues, but was undetectable in noncancerous tissue. Immunohistochemistry was performed to directly show expression of COX-2 in tumor tissue.
CONCLUSION
These results suggest that COX-2 may be an important factor for esophageal cancer and inhibition of COX-2 may be helpful for prevention and possibly treatment of this cancer.
目的
确定环氧化酶-2（COX-2）是否在人食管鳞状细胞癌中表达。
方法
采用定量逆转录-聚合酶链反应（RT-PCR）、蛋白质免疫印迹法、免疫组织化学和免疫荧光法评估食管组织中COX-2的表达水平。
结果
与相邻的非癌组织相比，食管鳞状细胞癌中COX-2 mRNA水平增加了80多倍。30例食管鳞状细胞癌组织中有21例存在COX-2蛋白，但在非癌组织中未检测到。进行免疫组织化学以直接显示COX-2在肿瘤组织中的表达。
结论
这些结果表明，COX-2可能是食管癌的一个重要因素，抑制COX-2可能有助于预防和治疗这种癌症。
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