Liu X, Li P, Zhang S-T, You H, Jia J-D, Yu Z-L
Beijing Friendship Hospital, Capital Medical University, Beijing Digestive Diseases Center, Beijing, China.
Dis Esophagus. 2008;21(1):9-14. doi: 10.1111/j.1442-2050.2007.00697.x.
To investigate cyclooxygenase-2 (COX-2) mRNA expression in human esophageal squamous cell carcinoma and the effect of a non-steroidal anti-inflammatory drug (NSAID) on it, in order to explore the mechanism of COX-2 in esophageal squamous cell carcinoma (ESCC) carcinogenesis and the ability of NSAID to prevent or treat ESCC. Frozen specimens of human ESCC and adjacent normal esophageal squamous epithelium pairs (n = 22) were examined for COX-2 mRNA expression by reverse-transcription polymerase chain reaction (RT-PCR). After incubation with aspirin (a non-selective COX inhibitor) or Nimesulide (a selective COX-2 inhibitor), the proliferation status of two human esophageal squamous cancer cell lines, EC-9706 and EC-109, was quantified by 3-(4,5-dimethyl-thiazol-2yl)-2,5-diphenyltetrazolium bromide assay. The expression of COX-2 mRNA in these cells was detected by RT-PCR. COX-2 mRNA was expressed in 12 of 22 (54.5%) ESCC tissue samples, but it was undetectable in all the specimens of adjacent normal esophageal squamous epithelium COX-2 mRNA expression. Both aspirin (5-20 mmol/L) and Nimesulide (0.1-0.8 mmol/L) inhibited EC-9706 cell line proliferation and suppressed its COX-2 mRNA expression dose-dependently. However, only aspirin (5-20 mmol/L) could inhibit proliferation in the EC-109 cell line and suppress COX-2 mRNA expression. Nimesulide (0.1-0.8 mmol/L) could neither inhibit EC-109 cell growth nor suppress COX-2 mRNA expression. COX-2 mRNA expression is a frequent phenomenon in human ESCC tissue samples and plays an important role in the carcinogenesis of ESCC. NSAID may be useful in the chemoprevention and therapy of human ESCC and its effects are likely to be mediated by modulating COX-2 activity.
为研究环氧化酶-2（COX-2）mRNA在人食管鳞状细胞癌中的表达情况以及非甾体抗炎药（NSAID）对其的影响，以探讨COX-2在食管鳞状细胞癌（ESCC）发生发展中的作用机制以及NSAID预防或治疗ESCC的能力。采用逆转录聚合酶链反应（RT-PCR）检测22对人ESCC及其癌旁正常食管鳞状上皮的冰冻标本中COX-2 mRNA的表达。用阿司匹林（一种非选择性COX抑制剂）或尼美舒利（一种选择性COX-2抑制剂）孵育后，采用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐法检测两种人食管鳞状癌细胞系EC-9706和EC-109的增殖状态。通过RT-PCR检测这些细胞中COX-2 mRNA的表达。COX-2 mRNA在22例ESCC组织标本中的12例（54.5%）中表达，但在所有癌旁正常食管鳞状上皮标本中均未检测到COX-2 mRNA表达。阿司匹林（5 - 20 mmol/L）和尼美舒利（0.1 - 0.8 mmol/L）均能剂量依赖性地抑制EC-9706细胞系增殖并抑制其COX-2 mRNA表达。然而，只有阿司匹林（5 - 20 mmol/L）能抑制EC-109细胞系增殖并抑制COX-2 mRNA表达。尼美舒利（0.1 - 0.8 mmol/L）既不能抑制EC-109细胞生长，也不能抑制COX-2 mRNA表达。COX-2 mRNA表达在人ESCC组织标本中较为常见，在ESCC的发生发展中起重要作用。NSAID可能对人ESCC的化学预防和治疗有用，其作用可能通过调节COX-2活性介导。
