Zhang Jian, Yang Zhuo, Wu Samuel M
Cullen Eye Institute, Baylor College of Medicine. One Baylor Plaza, Houston 77030, USA.
Vis Neurosci. 2004 Mar-Apr;21(2):157-66. doi: 10.1017/s0952523804042075.
In the present study, using double- or triple-label immunocytochemistry in conjunction with confocal microscopy, we aimed to examine the population and distribution of photoreceptors, GABAergic and glycinergic amacrine cells, and ganglion cells, which are basic but important parameters for studying the structure-function relationship of the salamander retina. We found that the outer nuclear layer (ONL) contained 82,019 +/- 3203 photoreceptors, of which 52% were rods and 48% were cones. The density of photoreceptors peaked at approximately 8000 cells/mm2 in the ventral and dropped to approximately 4000 cells/mm2 in the dorsal retina. In addition, the rod/cone ratio was less than 1 in the central retina but larger than I in the periphery. Moreover, in the proximal region of the inner nuclear layer (INL3), the total number of cells was 50,576 +/- 8400. GABAergic and glycinergic amacrine cells made up approximately 78% of all cells in this layer, including 43% GABAergic, 32% glycinergic, and 3% GABA/glycine colocalized amacrine cells. The density of these amacrine cells was approximately 6500 cells/mm2 in the ventral and approximately 3200 cells/mm2 in the dorsal area. The ratio of GABAergic to glycinergic amacrine cells was larger than 1. Furthermore, in the ganglion cell layer (GCL), among a total of 36,007 +/- 2010 cells, ganglion cells accounted for 65.7 +/- 1.5% of the total cells, whereas displaced GABAergic and glycinergic amacrine cells comprised about 4% of the cells in this layer. The ganglion cell density was approximately 1800 cells/mm2 in the ventral and approximately 600 cells/mm2 in the dorsal retina. Our data demonstrate that all three major cell types are not uniformly distributed across the salamander retina. Instead, they exhibit a higher density in the ventral than in the dorsal retina and their spatial arrangement is associated with the retinal topography. These findings provide a basic anatomical reference for the electrophysiological study of this species.
在本研究中，我们利用双重或三重标记免疫细胞化学结合共聚焦显微镜，旨在检测光感受器、γ-氨基丁酸（GABA）能和甘氨酸能无长突细胞以及神经节细胞的数量和分布，这些是研究蝾螈视网膜结构-功能关系的基本但重要的参数。我们发现，外核层（ONL）包含82,019±3203个光感受器，其中52%为视杆细胞，48%为视锥细胞。光感受器密度在腹侧约8000个细胞/mm²处达到峰值，在背侧视网膜降至约4000个细胞/mm²。此外，中央视网膜的视杆/视锥细胞比率小于1，而周边区域则大于1。此外，在内核层近端区域（INL3），细胞总数为50,576±8400个。GABA能和甘氨酸能无长突细胞约占该层所有细胞的78%，其中43%为GABA能，32%为甘氨酸能，3%为GABA/甘氨酸共定位无长突细胞。这些无长突细胞的密度在腹侧约为6500个细胞/mm²，在背侧区域约为3200个细胞/mm²。GABA能与甘氨酸能无长突细胞的比率大于1。此外，在神经节细胞层（GCL）中，在总共36,007±2010个细胞中，神经节细胞占总细胞数的65.7±1.5%，而移位的GABA能和甘氨酸能无长突细胞约占该层细胞的4%。神经节细胞密度在腹侧视网膜约为1800个细胞/mm²，在背侧视网膜约为600个细胞/mm²。我们的数据表明，这三种主要细胞类型在蝾螈视网膜中并非均匀分布。相反，它们在腹侧的密度高于背侧视网膜，并且它们的空间排列与视网膜地形图相关。这些发现为该物种的电生理研究提供了基本的解剖学参考。
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