Pham John W, Sontheimer Erik J
Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, IL 60208, USA.
Mol Cell. 2004 Jul 23;15(2):163-4. doi: 10.1016/j.molcel.2004.07.002.
An array of gene silencing pathways share a common early step: Dicer cleaves double-stranded RNA (dsRNA) into approximately 20-25 nucleotide fragments that direct the silencing machinery to specific targets. A recent report in Cell reveals how Dicer's two RNase III domains collaborate during dsRNA processing and sets the stage for a deeper understanding of Dicer's roles in later phases of silencing complex assembly.
Dicer将双链RNA（dsRNA）切割成大约20 - 25个核苷酸的片段，这些片段将沉默机制导向特定靶点。《细胞》杂志最近的一篇报道揭示了Dicer的两个RNase III结构域在dsRNA加工过程中是如何协作的，并为更深入理解Dicer在沉默复合体组装后期阶段的作用奠定了基础。
