包膜噬菌体phi6磷脂的起源

The origin of phospholipids of the enveloped bacteriophage phi6.

作者信息

Laurinavicius Simonas, Käkelä Reijo, Bamford Dennis H, Somerharju Pentti

机构信息

Department of Biochemistry, Institute of Biomedicine, University of Helsinki, 00014, Helsinki, Finland.

出版信息

Virology. 2004 Aug 15;326(1):182-90. doi: 10.1016/j.virol.2004.05.021.

DOI:10.1016/j.virol.2004.05.021

PMID:15262506

Abstract

The phospholipid class and molecular species compositions of bacteriophage phi6 and its host Pseudomonas syringae were determined quantitatively using TLC and liquid-chromatography/electrospray ionization mass-spectrometry. In addition, the fatty acid compositions of the phospholipids were analyzed by gas-chromatography/mass-spectrometry. The phage contained significantly more phosphatidylglycerol (PG) and less phosphatidylethanolamine (PE) than the host cytoplasmic (CM) and outer (OM) membranes. In addition, the phospholipid molecular species composition of the viral membrane differed from those of the host membranes, but resembled that of CM more than OM as shown by principal component analysis (PCA). The membrane of phi6 contained more 34:1 and 34:2, and less 32:1 PE and PG molecular species than the host CM or OM. Also, phi6 contained negligible amounts of saturated phospholipid molecular species. These data provide the first biochemical evidence suggesting that phi6 obtains its lipids from the CM. This process is not unselective, but certain phospholipid species are preferentially incorporated in the phage membrane. Common factors leading to similar enrichment of PG in every membrane-containing bacterial virus system studied so far (phi6, PM2, PRD1, PR4, Bam35) are discussed.

摘要

使用薄层色谱法（TLC）和液相色谱/电喷雾电离质谱法对噬菌体phi6及其宿主丁香假单胞菌的磷脂类别和分子种类组成进行了定量测定。此外，通过气相色谱/质谱法分析了磷脂的脂肪酸组成。与宿主细胞质膜（CM）和外膜（OM）相比，噬菌体中磷脂酰甘油（PG）含量显著更高，而磷脂酰乙醇胺（PE）含量更低。此外，病毒膜的磷脂分子种类组成与宿主膜不同，但主成分分析（PCA）表明，与外膜相比，它更类似于细胞质膜。phi6的膜中34:1和34:2的含量比宿主细胞质膜或外膜更多，而32:1的PE和PG分子种类比宿主细胞质膜或外膜更少。此外，phi6中饱和磷脂分子种类的含量可忽略不计。这些数据提供了首个生化证据，表明phi6从细胞质膜获取其脂质。这一过程并非无选择性，而是某些磷脂种类优先掺入噬菌体膜中。本文讨论了导致目前所研究的每个含膜细菌病毒系统（phi6、PM2、PRD1、PR4、Bam35）中PG出现类似富集现象的共同因素。