Oursel Delphine, Loutelier-Bourhis Corinne, Orange Nicole, Chevalier Sylvie, Norris Victor, Lange Catherine M
Spectrométrie de Masse Bio-Organique, CNRS UMR 6014, Université de Rouen, 76821 Mont-Saint-Aignan cedex, France.
Rapid Commun Mass Spectrom. 2007;21(11):1721-8. doi: 10.1002/rcm.3013.
A liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) method using reversed-phase chromatography was developed for the analysis of phospholipids from bacterial extracts of a wild-type strain of Escherichia coli. Product ion mass spectra from M--H precursor ions allowed an identification of individual phospholipid species that includes both fatty acid composition and fatty acyl location on the glycerol backbone using diagnostic product ions. Thus, complete assignment, including sn-1/sn-2 fatty acyl position, was achieved for this strain of E. coli. In addition, the phospholipids were quantified relative to one another using an internal standard method.
建立了一种使用反相色谱的液相色谱/电喷雾电离串联质谱（LC/ESI-MS/MS）方法，用于分析野生型大肠杆菌菌株细菌提取物中的磷脂。来自M-H前体离子的产物离子质谱图，通过诊断性产物离子，能够鉴定包括脂肪酸组成和甘油主链上脂肪酰基位置的单个磷脂种类。因此，实现了对该大肠杆菌菌株包括sn-1/sn-2脂肪酰基位置的完整归属。此外，使用内标法对磷脂进行了相互定量。
