在假单胞菌M18中，吩嗪-1-羧酸受gacA负调控，而绿脓菌素受gacA正调控。
Phenazine-1-carboxylic acid is negatively regulated and pyoluteorin positively regulated by gacA in Pseudomonas sp. M18.
作者信息
Ge Yihe, Huang Xianqing, Wang Sulian, Zhang Xuehong, Xu Yuquan
机构信息
College of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai 200240, PR China.
出版信息
FEMS Microbiol Lett. 2004 Aug 1;237(1):41-7. doi: 10.1016/j.femsle.2004.06.028.
The biosynthesis of antimicrobial metabolites is controlled by the GacS/GacA two-component regulatory system in Pseudomonas species. The production of phenazine-1-carboxylic acid and pyoluteorin is differentially regulated by GacA in Pseudomonas sp. M18. Pyoluteorin was reduced to nondetectable level in culture of the gacA insertional mutant strain M18G grown in King's medium B broth, whereas phenazine-1-carboxylic acid production was increased 30-fold over that of the wild-type strain. Production of both antibiotics was restored to wild-type levels after complementation in trans with the wild-type gacA gene. Expression of the translational fusions phzA'-'lacZ and pltA'-'lacZ confirmed the effect of GacA on both biosynthetic operons.
假单胞菌属中抗菌代谢物的生物合成受GacS/GacA双组分调控系统控制。在假单胞菌M18中，吩嗪-1-羧酸和绿脓菌素的产生受GacA的差异调控。在King's培养基B肉汤中生长的gacA插入突变株M18G的培养物中，绿脓菌素降至无法检测的水平，而吩嗪-1-羧酸的产量比野生型菌株增加了30倍。用野生型gacA基因进行反式互补后，两种抗生素的产量恢复到野生型水平。翻译融合体phzA'-'lacZ和pltA'-'lacZ的表达证实了GacA对两个生物合成操纵子的作用。
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