利用基于马铃薯X病毒的农杆菌介导瞬时表达系统在本氏烟草中表达人乳铁蛋白N端结构域

Expression of a human lactoferrin N-lobe in Nicotiana benthmiana with potato virus X-based agroinfection.

作者信息

Li Yong, Geng Yunfeng, Song Houhui, Zheng Guangyu, Huan Liandong, Qiu Bingsheng

机构信息

Molecular Microbiological Centre, Institute of Microbiology, Chinese Academy of Sciences, Beijing 10080, P.R. China.

出版信息

Biotechnol Lett. 2004 Jun;26(12):953-7. doi: 10.1023/b:bile.0000030038.27358.20.

DOI:10.1023/b:bile.0000030038.27358.20

PMID:15269518

Abstract

A DNA fragment encoding the N-terminal half (N-lobe) of the human lactoferrin (hLfN) gene has now been cloned into recombinant Potexvirus potato virus X (PVX) vector and expressed in Nicotiana benthmiana using agroinfection. Western blot analysis showed the recombinant protein with an apparent molecular mass on electrophoresis of ca. 40 kDa, corresponding to the predicted size of the hLfN. The yield of hLfN reached a maximum (up to 0.6% of total soluble proteins) when recombinant PVX systemically infected an entire plant. Protein extracts from infected plants had antibacterial activity.

摘要

编码人乳铁蛋白（hLfN）基因N端半段（N叶）的DNA片段现已克隆到重组马铃薯X病毒（PVX）载体中，并通过农杆菌介导的感染在本氏烟草中表达。蛋白质印迹分析显示，重组蛋白在电泳中的表观分子量约为40 kDa，与hLfN的预测大小相符。当重组PVX系统感染整株植物时，hLfN的产量达到最高（占总可溶性蛋白的0.6%）。感染植物的蛋白提取物具有抗菌活性。