Konadaka Sri Rajaravindra, Balasamudram Chandrasekhar Parthasarathi, Sirisilla Architha, Mohammed Qudrathulla Khan Quadri, Jakkoju Thejasri, Bheemreddy Rajith Reddy, Bhattacharya Tarun Kumar, Ullengala Rajkumar, Chatterjee Rudra Nath
ICAR-Directorate of Poultry Research, Hyderabad 500030, India.
ICAR-National Research Centre on Equines, Hisar 125001, India.
Pharmaceutics. 2025 Aug 7;17(8):1023. doi: 10.3390/pharmaceutics17081023.
Lactoferrin (LF) is an 80 kDa iron-binding glycoprotein primarily found in milk, saliva, tears, and nasal secretions. LF is well known for its antibacterial and immunomodulatory effects. However, the extraction of LF from milk is inadequate for large-scale therapeutic applications, presenting a challenge for economic mass production. Recombinant protein expression systems offer a solution to overcome this challenge and efficient production of LF. This review discusses recent progress in the translational research of LF gene transfer and biopharming, focusing on different expression systems such as bacteria, yeast, filamentous fungi, transgenic crops, and animals as well as purification methods. The optimization of expression yields, prospects for genetic engineering, and biotechnology to enhance LF production for biomedical applications are emphasized. This review systematically sourced the literature from 1987 to 2025 from leading scientific databases, including PubMed, Scopus, Web of Science, and Google Scholar. Despite ongoing debates, progress in this field indicates a viable path towards the effective use of LF in therapeutic settings.
乳铁蛋白（LF）是一种80千道尔顿的铁结合糖蛋白，主要存在于牛奶、唾液、眼泪和鼻分泌物中。LF以其抗菌和免疫调节作用而闻名。然而，从牛奶中提取LF不足以满足大规模治疗应用的需求，这给经济批量生产带来了挑战。重组蛋白表达系统为克服这一挑战和高效生产LF提供了一种解决方案。本文综述了LF基因转移和生物制药转化研究的最新进展，重点介绍了细菌、酵母、丝状真菌、转基因作物和动物等不同表达系统以及纯化方法。强调了优化表达产量、基因工程前景以及生物技术以提高LF产量用于生物医学应用的重要性。本文系统地从1987年至2025年的主要科学数据库中检索文献，包括PubMed、Scopus、Web of Science和谷歌学术。尽管仍有争议，但该领域的进展表明了在治疗环境中有效使用LF的可行途径。
