使用BacLight对酿酒酵母活力进行定量分析。
Quantification of Saccharomyces cerevisiae viability using BacLight.
作者信息
Zhang Tong, Fang Herbert H P
机构信息
Environmental Biotechnology Laboratory, Department of Civil Engineering The University of Hong Kong, Pokfulam Road, Hong Kong SAR, China.
出版信息
Biotechnol Lett. 2004 Jun;26(12):989-92. doi: 10.1023/b:bile.0000030045.16713.19.
Yeast viability can be accurately quantified using BacLight, a kit which so far has been used only for bacterial analysis. Upon staining, viable cells can be differentiated from non-viable ones by either confocal laser scanning microscopy (CLSM), epifluorescence microscopy, or flow cytometry. Using Saccharomyces cerevisiae as a model, viabilities quantified by CLSM deviated an average of 1.7% from the actual data, and those determined by flow-cytometry by 1.4%.
使用BacLight可以准确量化酵母活力，该试剂盒迄今为止仅用于细菌分析。染色后，通过共聚焦激光扫描显微镜（CLSM）、落射荧光显微镜或流式细胞术可区分活细胞和非活细胞。以酿酒酵母为模型，CLSM量化的活力与实际数据平均偏差1.7%，流式细胞术测定的活力与实际数据平均偏差1.4%。
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