Comas-Riu Jaume, Rius Núria
Serveis Cientificotècnics, Universitat de Barcelona, 08028 Barcelona, Spain.
J Ind Microbiol Biotechnol. 2009 Aug;36(8):999-1011. doi: 10.1007/s10295-009-0608-x. Epub 2009 Jun 26.
Flow cytometry has become a valuable tool in food microbiology. By analysing large numbers of cells individually using light-scattering and fluorescence measurements, this technique reveals both cellular characteristics and the levels of cellular components. Flow cytometry has been developed to rapidly enumerate microorganisms; to distinguish between viable, metabolically active and dead cells, which is of great importance in food development and food spoilage; and to detect specific pathogenic microorganisms by conjugating antibodies with fluorochromes, which is of great use in the food industry. In addition, high-speed multiparametric data acquisition, analysis and cell sorting, which allow other characteristics of individual cells to be studied, have increased the interest of food microbiologists in this technique. This mini-review gives an overview of the principles of flow cytometry and examples of the application of this technique in the food industry.
流式细胞术已成为食品微生物学中的一项重要工具。通过利用光散射和荧光测量对大量细胞进行逐个分析，该技术能够揭示细胞特征以及细胞成分的水平。流式细胞术已发展用于快速计数微生物；区分活细胞、代谢活跃细胞和死细胞，这在食品开发和食品变质过程中极为重要；以及通过将抗体与荧光染料结合来检测特定的致病微生物，这在食品工业中具有很大用途。此外，高速多参数数据采集、分析和细胞分选能够研究单个细胞的其他特征，这增加了食品微生物学家对该技术的兴趣。本综述简要介绍了流式细胞术的原理以及该技术在食品工业中的应用实例。
