含有2-乙酰氨基-2-脱氧-α-D-半乳糖磷酸的变形杆菌O34血清群O-多糖的结构

Structure of the O-polysaccharide of Proteus serogroup O34 containing 2-acetamido-2-deoxy-alpha-D-galactosyl phosphate.

作者信息

Perepelov Andrei V, Kołodziejska Katarzyna, Kondakova Anna N, Wykrota Marianna, Knirel Yuriy A, Sidorczyk Zygmunt, Rozalski Antoni

机构信息

N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect 47, 119991 Moscow, Russian Federation.

出版信息

Carbohydr Res. 2004 Aug 23;339(12):2145-9. doi: 10.1016/j.carres.2004.06.005.

DOI:10.1016/j.carres.2004.06.005

PMID:15280059

Abstract

On mild acid degradation of the lipopolysaccharide of Proteus vulgaris O34, strain CCUG 4669, the O-polysaccharide was cleaved at a glycosyl-phosphate linkage that is present in the main chain. The resultant phosphorylated oligosaccharides and an alkali-treated lipopolysaccharide were studied by sugar and methylation analyses along with 1H and 13C NMR spectroscopy, and the following structure of the branched tetrasaccharide phosphate repeating unit of the O-polysaccharide was established: [carbohydrate structure: see text]The O-polysaccharide of Proteus mirabilis strain TG 276 was found to have the same structure and, based on the structural and serological data, this strain was proposed to be classified into the same Proteus serogroup O34.

摘要

在普通变形杆菌O34（CCUG 4669菌株）脂多糖的温和酸降解过程中，O-多糖在主链中存在的糖基磷酸键处被切断。通过糖分析、甲基化分析以及¹H和¹³C NMR光谱对所得的磷酸化寡糖和碱处理的脂多糖进行了研究，并确定了O-多糖的分支四糖磷酸重复单元的以下结构：[碳水化合物结构：见正文]奇异变形杆菌TG 276菌株的O-多糖被发现具有相同的结构，并且基于结构和血清学数据，该菌株被提议归入同一变形杆菌血清群O34。