Zabłotni Agnieszka, Perepelov Andrei V, Knirel Yuriy A, Sidorczyk Zygmunt
Institute of Microbiology and Immunology, University of Lodz, 90-237 Lodz, Poland.
Carbohydr Res. 2005 Aug 15;340(11):1908-13. doi: 10.1016/j.carres.2005.05.014.
A neutral O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Proteus mirabilis OC (CCUG 10702) and studied by sugar and methylation analyses and (1)H and (13)C NMR spectroscopy. The following structure of the tetrasaccharide repeating unit of the polysaccharide was established: [structure: see text]. Based on the unique structure of the O-polysaccharide and serological data, we propose classifying P. mirabilis OC (CCUG 10702) into a new separate Proteus serogroup O75. A weak cross-reaction of O-antiserum against P. mirabilis OC with the lipopolysaccharide of P. mirabilis O49 was accounted for by a similarity in the O-polysaccharide structures.
通过对奇异变形杆菌OC(CCUG 10702)脂多糖进行温和酸降解获得了一种中性O-多糖,并通过糖分析、甲基化分析以及¹H和¹³C NMR光谱对其进行了研究。确定了该多糖四糖重复单元的以下结构:[结构:见正文]。基于O-多糖的独特结构和血清学数据,我们建议将奇异变形杆菌OC(CCUG 10702)归类为一个新的独立变形杆菌血清群O75。O-抗血清对奇异变形杆菌OC与奇异变形杆菌O49脂多糖的弱交叉反应是由O-多糖结构的相似性引起的。