Moehring Amanda J, Mackay Trudy F C
Department of Genetics, North Carolina State University, Raleigh, North Carolina 27695-7614, USA.
Genetics. 2004 Jul;167(3):1249-63. doi: 10.1534/genetics.103.024372.
Male mating behavior is an important component of fitness in Drosophila and displays segregating variation in natural populations. However, we know very little about the genes affecting naturally occurring variation in mating behavior, their effects, or their interactions. Here, we have mapped quantitative trait loci (QTL) affecting courtship occurrence, courtship latency, copulation occurrence, and copulation latency that segregate between a D. melanogaster strain selected for reduced male mating propensity (2b) and a standard wild-type strain (Oregon-R). Mating behavior was assessed in a population of 98 recombinant inbred lines derived from these two strains and QTL affecting mating behavior were mapped using composite interval mapping. We found four QTL affecting male mating behavior at cytological locations 1A;3E, 57C;57F, 72A;85F, and 96F;99A. We used deficiency complementation mapping to map the autosomal QTL with much higher resolution to five QTL at 56F5;56F8, 56F9;57A3, 70E1;71F4, 78C5;79A1, and 96F1;97B1. Quantitative complementation tests performed for 45 positional candidate genes within these intervals revealed 7 genes that failed to complement the QTL: eagle, 18 wheeler, Enhancer of split, Polycomb, spermatocyte arrest, l(2)05510, and l(2)k02206. None of these genes have been previously implicated in mating behavior, demonstrating that quantitative analysis of subtle variants can reveal novel pleiotropic effects of key developmental loci on behavior.
雄性交配行为是果蝇适应性的一个重要组成部分，并且在自然种群中表现出分离变异。然而，我们对影响交配行为自然变异的基因、它们的作用或相互作用知之甚少。在这里，我们定位了影响求偶发生、求偶潜伏期、交配发生和交配潜伏期的数量性状基因座（QTL），这些性状在一个因雄性交配倾向降低而被选择的黑腹果蝇品系（2b）和一个标准野生型品系（俄勒冈 - R）之间分离。在由这两个品系衍生的98个重组自交系群体中评估了交配行为，并使用复合区间作图法对影响交配行为的QTL进行了定位。我们在细胞学位置1A;3E、57C;57F、72A;85F和96F;99A发现了四个影响雄性交配行为的QTL。我们使用缺失互补作图法将常染色体QTL以更高的分辨率定位到五个QTL，分别位于56F5;56F8、56F9;57A3、70E1;71F4、78C5;79A1和96F1;97B1。对这些区间内的45个位置候选基因进行的定量互补测试揭示了7个不能互补QTL的基因：鹰基因、18轮车基因、分裂增强子、多梳蛋白、精母细胞停滞基因、l(2)05510和l(2)k02206。这些基因以前都没有被认为与交配行为有关，这表明对细微变异的定量分析可以揭示关键发育基因座对行为的新的多效性作用。
