在构巢曲霉中，GanB Gα蛋白对无性孢子形成起负调控作用，并在分生孢子萌发中发挥正性作用。
The GanB Galpha-protein negatively regulates asexual sporulation and plays a positive role in conidial germination in Aspergillus nidulans.
作者信息
Chang Mi-Hee, Chae Keon-Sang, Han Dong-Min, Jahng Kwang-Yeop
机构信息
Division of Biological Sciences, Chonbuk National University, Chonju 561-756, Republic of Korea.
出版信息
Genetics. 2004 Jul;167(3):1305-15. doi: 10.1534/genetics.103.025379.
Abstract
We isolated the ganB gene encoding the Galpha-protein homolog from Aspergillus nidulans. To investigate the cellular function of GanB, various mutant strains were isolated. Deletion of constitutively inactive ganB mutants showed conidiation and derepressed brlA expression in a submerged culture. Constitutive activation of GanB caused a reduction in hyphal growth and a severe defect in asexual sporulation. We therefore propose that GanB may negatively regulate asexual sporulation through the BrlA pathway. In addition, deletion or constitutive inactivation of GanB reduced germination rate while constitutive activation led to precocious germination. Furthermore, conidia of a constitutively active mutant could germinate even without carbon source. Taken together, these results indicated that GanB plays a positive role during germination, possibly through carbon source sensing, and negatively regulates asexual conidiation in A. nidulans.
摘要
我们从构巢曲霉中分离出了编码Gα蛋白同源物的ganB基因。为了研究GanB的细胞功能，我们分离出了各种突变菌株。组成型无活性ganB突变体的缺失在深层培养中显示出分生孢子形成并解除了brlA表达的抑制。GanB的组成型激活导致菌丝生长减少和无性孢子形成严重缺陷。因此，我们认为GanB可能通过BrlA途径负调控无性孢子形成。此外，GanB的缺失或组成型失活降低了萌发率，而组成型激活则导致早熟萌发。此外，组成型活性突变体的分生孢子即使在没有碳源的情况下也能萌发。综上所述，这些结果表明GanB在萌发过程中发挥着积极作用，可能是通过碳源感知，并且在构巢曲霉中负调控无性分生孢子形成。
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