Van Aken Benoit, Peres Caroline M, Doty Sharon Lafferty, Yoon Jong Moon, Schnoor Jerald L
Department of Civil and Environmental Engineering, University of Iowa, 4105 Seamans Center, Iowa City, IA 52242, USA.
Department of Microbiology, University of Iowa, 3-432 Bowen Science Building, Iowa City, IA 52242, USA.
Int J Syst Evol Microbiol. 2004 Jul;54(Pt 4):1191-1196. doi: 10.1099/ijs.0.02796-0.
A pink-pigmented, aerobic, facultatively methylotrophic bacterium, strain BJ001T, was isolated from internal poplar tissues (Populus deltoidesxnigra DN34) and identified as a member of the genus Methylobacterium. Phylogenetic analyses showed that strain BJ001T is related to Methylobacterium thiocyanatum, Methylobacterium extorquens, Methylobacterium zatmanii and Methylobacterium rhodesianum. However, strain BJ001T differed from these species in its carbon-source utilization pattern, particularly its use of methane as the sole source of carbon and energy, an ability that is shared with only one other member of the genus, Methylobacterium organophilum. In addition, strain BJ001T is the only member of the genus Methylobacterium to be described as an endophyte of poplar trees. On the basis of its physiological, genotypic and ecological properties, the isolate is proposed as a member of a novel species of the genus Methylobacterium, Methylobacterium populi sp. nov. (type strain, BJ001T=ATCC BAA-705T=NCIMB 13946T).
从杨树（Populus deltoidesxnigra DN34）内部组织中分离出一株粉红色色素、需氧、兼性甲基营养型细菌，BJ001T菌株，鉴定为甲基杆菌属成员。系统发育分析表明，BJ001T菌株与硫氰酸甲基杆菌、扭脱甲基杆菌、扎特曼甲基杆菌和罗得西亚甲基杆菌相关。然而，BJ001T菌株在碳源利用模式上与这些物种不同，特别是其以甲烷作为唯一碳源和能源的利用能力，该属中只有嗜有机甲基杆菌的另一个成员具有此能力。此外，BJ001T菌株是甲基杆菌属中唯一被描述为杨树内生菌的成员。基于其生理、基因型和生态特性，该分离株被提议作为甲基杆菌属一个新物种——杨树甲基杆菌（Methylobacterium populi sp. nov.）的成员（模式菌株，BJ001T=ATCC BAA-705T=NCIMB 13946T）。
