Alexandrakis M G, Passam F H, Dambaki C, Pappa C A, Stathopoulos E N
Department of Haematology, University Hospital of Heraklion, PO Box 1352, 71110, Heraklion, Crete, Greece.
J Clin Pathol. 2004 Aug;57(8):856-60. doi: 10.1136/jcp.2003.013110.
Angiogenesis correlates with disease progression in various haematological malignancies. This study investigated the association between microvascular density (MVD) and the Ki-67 proliferation index (Ki-67 PI), bone marrow infiltration, and C reactive protein (CRP) in patients with multiple myeloma.
Bone marrow MVD was examined in 44 biopsies at diagnosis and 15 in plateau phase by immunostaining the endothelial cells with a monoclonal antibody to CD34. The Ki-67 PI was evaluated by a double immunostaining technique using the monoclonal antibodies MIB-1 and CD38.
MVD, Ki-67 PI, bone marrow infiltration, and CRP were significantly higher in pretreatment patients than in controls and decreased in patients achieving plateau phase. MVD significantly correlated with Ki-67 PI and infiltration, and Ki-67 correlated with infiltration.
In multiple myeloma, apart from being a marker of proliferative activity, Ki-67 is also associated with bone marrow angiogenesis and tumour burden.
血管生成与多种血液系统恶性肿瘤的疾病进展相关。本研究调查了多发性骨髓瘤患者微血管密度（MVD）与Ki-67增殖指数（Ki-67 PI）、骨髓浸润及C反应蛋白（CRP）之间的关联。
通过用抗CD34单克隆抗体对内皮细胞进行免疫染色，在44例诊断时的骨髓活检标本及15例平台期标本中检测骨髓MVD。采用MIB-1和CD38单克隆抗体的双重免疫染色技术评估Ki-67 PI。
预处理患者的MVD、Ki-67 PI、骨髓浸润及CRP显著高于对照组，且在达到平台期的患者中降低。MVD与Ki-67 PI及浸润显著相关，且Ki-67与浸润相关。
在多发性骨髓瘤中，Ki-67除作为增殖活性标志物外，还与骨髓血管生成及肿瘤负荷相关。
