Putney James W
National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Post Office Box 12233, Research Triangle Park, NC 27709, USA.
Sci STKE. 2004 Jul 20;2004(243):pe37. doi: 10.1126/stke.2432004pe37.
A major mechanism whereby calcium entry into cells is regulated is the store-operated or capacitative calcium entry pathway. In this article, two basic issues are discussed: (i) the methods investigators use to measure store-operated entry, and (ii) the role played by the store-operated pathway in responses to hormones and neurotransmitters under physiological conditions. The two topics are considered together because they are closely interrelated; as we begin to ask questions about calcium movements at low concentrations of agonists, the technology to measure these movements becomes increasing challenging.
钙离子进入细胞的一种主要调节机制是储存-操纵性或容量性钙离子内流途径。在本文中，讨论了两个基本问题：（i）研究人员用于测量储存-操纵性内流的方法，以及（ii）在生理条件下，储存-操纵性途径在对激素和神经递质的反应中所起的作用。将这两个主题放在一起考虑是因为它们密切相关；当我们开始询问在低浓度激动剂情况下钙离子的移动情况时，测量这些移动的技术变得越来越具有挑战性。
