Thirion Sylvie, Berenbaum Francis
UMR CNRS 6544, Laboratoire Interactions Cellulaires Neuroendocriennes, Institut Féderatif de Recherche Jean Roche, Faculté de Médecine Nord, Université Aix-Marseille II, Marseille, France.
Methods Mol Med. 2004;100:1-14. doi: 10.1385/1-59259-810-2:001.
The culture of chondrocytes is one of the most powerful tool for exploring the intracellular and molecular features of chondrocyte differentiation and activation. However, chondrocytes tend to dedifferentiate to fibroblasts when they are subcultured, which is a major problem. This chapter describes several protocols for culturing chondrocytes of different anatomical origins (articular and costal chondrocytes) from various species (humans, mice, rabbits, and cattle). All these protocols involve primary cultures in order to limit dedifferentiation. This chapter also describes a new protocol for culturing mouse articular chondrocytes.
软骨细胞培养是探索软骨细胞分化和激活的细胞内及分子特征的最有力工具之一。然而，软骨细胞传代培养时容易去分化为成纤维细胞，这是一个主要问题。本章描述了几种从不同物种（人类、小鼠、兔子和牛）培养不同解剖来源（关节软骨细胞和肋软骨细胞）软骨细胞的方法。所有这些方法都涉及原代培养，以限制去分化。本章还描述了一种培养小鼠关节软骨细胞的新方法。
