Melixetian Marina, Helin Kristian
European Institute of Oncology, Department of Experimental Oncology, Milan, Italy.
Cell Cycle. 2004 Aug;3(8):1002-4. Epub 2004 Aug 25.
Eukaryotes have evolved multiple mechanisms to restrict DNA replication to once per cell cycle. These mechanisms prevent relicensing of origins of replication after initiation of DNA replication in S phase until the end of mitosis. Most of our knowledge of mechanisms controlling prereplication complex (preRC) formation are based on studies from yeast and Xenopus, while much less is known for mammalian cells. Here we discuss our recent data demonstrating that Geminin is required for preventing rereplication in human normal and cancer cells.
真核生物已经进化出多种机制，以确保DNA复制在每个细胞周期中仅发生一次。这些机制可防止在S期DNA复制起始后复制起点的重新许可，直至有丝分裂结束。我们对控制复制前复合体（preRC）形成机制的了解大多基于酵母和非洲爪蟾的研究，而对哺乳动物细胞的了解则少得多。在此，我们讨论我们最近的数据，这些数据表明Geminin对于防止人类正常细胞和癌细胞中的再复制是必需的。
